Biolegend, 385202, Purified anti-human CD25 Antibody, 100μg

CD25 is a 55 kD transmembrane protein also known as interleukin-2 receptor alpha chain. CD25 in combination with CD122 and CD132 form a high affinity IL-2 receptor that binds to IL-2, playing a crucial role in activation of JAK1/JAK3 and STAT5 signaling. It is expressed on activated T cells as well as on CD4+ T regulatory cells, some progenitor B-cells and activated monocytes.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human CD25 transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone S20019D can block binding of clone M-A251 on target cells and is blocked by M-A251. Clone S20019D does not block binding of clones S20019C, BC96, and TÜ69 on target cells. Clone S20019D does not affect binding of IL-2 to the receptor. Clone S20019D shows comparable CD25 staining on 4% formaldehyde-fixed cells compared to unfixed.
RRID: AB_2936550 (BioLegend Cat. No. 385202)
Structure: 55 kD transmembrane protein
Distribution: Activated T cells, T regulatory cells, monocytes
Function: IL-2 receptor
Interaction: CD122, CD132
Ligand/Receptor: IL-2
Cell Type: Lymphocytes, T cells, Tregs
Biology Area: Cell Biology, Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. Waldmann T. 1991. J Biol Chem. 266:2681-4. Sakaguchi S, et al. 1995. The Journal of Immunology. 155:1151-64.
Gene ID: 3559
UniProt: View information about CD25 on UniProt.org
Clone: S20019D
Regulatory Status: RUO
Other Names: interleukin-2 receptor alpha chain, interluekin-2 receptor, IL2RA, IL-2Rα
Isotype: Mouse IgG1, κ