Biolegend, 385216, PE/Cyanine5 anti-human CD25 Antibody, 100tests

CD25 is a 55 kD transmembrane protein also known as interleukin-2 receptor alpha chain. CD25 in combination with CD122 and CD132 form a high affinity IL-2 receptor that binds to IL-2, playing a crucial role in activation of JAK1/JAK3 and STAT5 signaling. It is expressed on activated T cells as well as on CD4+ T regulatory cells, some progenitor B-cells and activated monocytes.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human CD25 transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine5 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Clone S20019D can block binding of clone M-A251 on target cells and is blocked by M-A251.Clone S20019D does not block binding of clones S20019C, BC96, and TÜ69 on target cells.Clone S20019D does not affect binding of IL-2 to the receptor. Clone S20019D shows comparable CD25 staining on 4% formaldehyde-fixed cells compared to unfixed.
RRID: AB_3675098 (BioLegend Cat. No. 385215) AB_3675098 (BioLegend Cat. No. 385216)
Structure: 55 kD transmembrane protein
Distribution: Activated T cells, T regulatory cells, monocytes
Function: IL-2 receptor
Interaction: CD122, CD132
Ligand/Receptor: IL-2
Cell Type: Lymphocytes, T cells, Tregs
Biology Area: Cell Biology, Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. Waldmann T. 1991. J Biol Chem. 266:2681-4. Sakaguchi S, et al. 1995. The Journal of Immunology. 155:1151-64.
Gene ID: 3559
UniProt: View information about CD25 on UniProt.org
Clone: S20019D
Regulatory Status: RUO
Other Names: interleukin-2 receptor alpha chain, interluekin-2 receptor, IL2RA, IL-2Rα
Isotype: Mouse IgG1, κ