Biolegend, 385402, Purified anti-human CD152 (CTLA-4) Recombinant Antibody, 100μg

CD152, also known as Cytotoxic T-Lymphocyte Antigen 4 (CTLA-4), is a 33 kD member of the immunoglobulin superfamily. It is transiently expressed on activated T cells. CTLA-4 is expressed on the surface of helper T cells and transmits an inhibitory signal to T cells. Regulatory T cells express high levels of CTLA-4. CTLA-4  is similar to CD28 in amino acid sequence, structure, and genomic organization. Whereas CD28 delivers a costimulatory signal in T cell activation, CTLA-4 negatively regulates cell-mediated immune responses through interaction with CD80 (B7-1) and CD86 (B7-2) present on antigen presenting cells (APC). CTLA-4 is thought to play a role in the induction and maintenance of immunological tolerance as well as the development of protective immunity and thymocyte regulation.
100μg
Verified Reactivity: Human
Antibody Type: Recombinant
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone QA20B10 can completely block the binding of parental clone to target cells.
RRID: AB_2936635 (BioLegend Cat. No. 385402)
Structure: Ig superfamily and 33 kD
Distribution: Activated T cells and B cells
Function: Negative regulator of T cell activation
Interaction: Antigen presenting cells
Ligand/Receptor: B7-1 (CD80), B7-2 (CD86)
Antigen References: Kuiper HM, et al. 1995. J Immunol. 155:1776-83. Pistillo MP, et al. 2003. Blood. 101:202-9. Tan PH, et al. 2005. Blood. 106:2936-43. Steiner K, et al. 2001. Clin Exp Immunol. 126:143-50.
Gene ID: 1493
UniProt: View information about CD152 on UniProt.org
Clone: QA20B10
Regulatory Status: RUO
Other Names: CTLA4, Cytotoxic T-Lymphocyte Antigen 4, CTLA-4
Isotype: Mouse IgG1, κ