Biolegend, 385817, APC/Cyanine7 anti-human CD7 Antibody, 25tests

CD7 is a 40 kD type I transmembrane glycoprotein also known as gp40. It is a member of the immunoglobulin superfamily found on T cells, NK cells, thymocytes, hematopoietic progenitors, and monocytes (weakly). CD7 is also expressed on acute lymphocytic leukemia (ALL) and some acute myeloid leukemia (AML) cells. CD7 crosslinking induces a calcium flux in T lymphocytes, presumably as a result of cytoplasmic domain association with PI3-kinase. CD7 costimulation can induce cytokine secretion and modulate cellular adhesion.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human CD7 recombinant protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC/Cyanine7 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool).
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: Clone A20153E can completely block binding of clone 4H9-CD7 and partially blocks binding of clone CD7-6B7 on target cells.
RRID: AB_3720513 (BioLegend Cat. No. 385817) AB_3720513 (BioLegend Cat. No. 385818)
Structure: Ig superfamily, type I transmembrane glycoprotein, 40 kD
Distribution: T cells, NK cells, thymocytes, hematopoietic progenitors, myeloid leukemic cell subsets
Function: T cell Activation
Cell Type: Hematopoietic stem and progenitors, Leukemia, NK cells, T cells, Thymocytes
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego. Stillwell R, et al. 2001. Immunol Res. 24:31-52. Rabinowich H, et al. 1994. J Immunol. 152:517-26.
Gene ID: 924
UniProt: View information about CD7 on UniProt.org
Clone: A20153E
Regulatory Status: RUO
Isotype: Mouse IgG2b, κ