Biolegend, 385908, Brilliant Violet 421™ anti-human IL-17A Recombinant Antibody, 100tests

IL-17A is the founding member of the IL-17 family, a group of six structurally related pro-inflammatory cytokines. IL-17A is the signature cytokine of Th17 subpopulation of CD4+ T cells. Neutrophils are also a major source of IL-17A. IL-17A can elicit multiple biological activities on a variety of cells. For instance, IL-17A can induce IL-6, IL-8, G-CSF, and PGE2 production from epithelial cells, endothelial cells, or fibroblast. IL-17A also further regulates neutrophil functions. Human IL-17A shares 63%, 63% and 72% amino acid sequence similarity to rat IL-17A, mouse IL-17A, and a protein encoded by the ORF13 gene of herpesvirus Saimiri (HVS) respectively.
100tests
Verified Reactivity: Human
Antibody Type: Recombinant
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Clone QA18A46 can completely block the binding of clone BL168 on target cells.
RRID: AB_3720539 (BioLegend Cat. No. 385907) AB_3720539 (BioLegend Cat. No. 385908)
Structure: ~15 kD cytokine
Distribution: Activated T cells, neutrophils
Function: Proinflammatory cytokine
Ligand/Receptor: IL-17A receptor
Cell Type: Lymphocytes, Neutrophils, T cells, Th17
Biology Area: Adaptive Immunity, Immunology, Innate Immunity
Molecular Family: Cytokines/Chemokines
Antigen References: Spriggs MK. 1997. J Clin Immunol. 17:366-9. Kawaguchi M, et al. 2004. J Allergy Clin Immunol. 114:1265-73. Furuzawa-Carballeda J, et al. 2007. Autoimmun Rev. 6:169-75.
Gene ID: 3605
UniProt: View information about IL-17A on UniProt.org
Clone: QA18A46
Regulatory Status: RUO
Other Names: Interluekin 17A, IL-17, CTLA8
Isotype: Mouse IgG1, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.