Biolegend, 386106, PE anti-human CD66c Antibody, 100tests

Carcinoembryonic antigen (CEA) Cell Adhesion Molecule 6 (CEACAM6) belongs to the (CEA) family whose members are glycosyl phosphatidyl inositol (GPI) anchored cell surface glycoproteins. As a cell adhesion molecule, CEACAM6 mediates homotypic binding with other CEA family members and heterotypic binding with integrin receptors. CEACAM6 is expressed on epithelial surfaces and in the myeloid lineage. Despite lacking transmembrane and intracellular domains, GPI-anchored proteins, including CEACAM family members, are able to influence transmembrane signaling via tyrosine kinases. CEACAM6 modulates c-Src tyrosine kinase activity. Overexpressed in more than 50% of all human adenocarcinomas, CEACAM6 promotes aberrant cell differentiation, anti-apoptosis, cell growth and resistance to therapeutic agents, cell invasion and metastasis.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant protein, aa 35-320
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_3068032 (BioLegend Cat. No. 386105) AB_3068032 (BioLegend Cat. No. 386106)
Structure: Glycosyl phosphatidyl inositol (GPI) anchored cell surface glycoproteins Immunoglobulin superfamily
Distribution: Apical Cell Membrane
Function: The extracellular N-terminus Ig-like V-type domain is necessary for homophilic and heterophilic intercellular adhesion.
Interaction: Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM5 and CEACAM8
Cell Type: Epithelial cells, Granulocytes
Biology Area: Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: Duxbury MS, et al. 2004. Oncogene. 23:465-73. Oikawa S, et al. 1991. J Biol Chem. 266:7995-8001. Noworolska A, et al. 1986. Cancer Detect Prev. 9:365-71. Wu S, et al. 2021. Transl Oncol. 14:101057
Gene ID: 4680
UniProt: View information about CD66c on UniProt.org
Clone: W20030E
Regulatory Status: RUO
Other Names: Carcinoembryonic antigen-related cell adhesion molecule 6
Isotype: Rat IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.