Biolegend, 386409, Alexa Fluor® 647 anti-human Glypican-4 Antibody, 25tests

Glypican-4 is a cell surface heparan sulfate proteoglycan that contains a core protein anchored to the cytoplasmic membrane via a GPI anchor. Glypicans function as coreceptors for a variety of growth factors including Wnt, bone morphogenetic proteins (BMPs), fibroblast growth factors, and Hedgehog. In particular, Glypican-4 enhances the Wnt3a dependent β-catenin pathway and the Wnt5a-dependent non-canonical Wnt signaling. Glypican-4 plays a role in the control of cell division and growth regulation and is upregulated in pancreatic cancer and colorectal cancer where it is involved in promoting chemoresistance. Glypican-4 also regulates glutamatergic synapse formation and function in the developing brain.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: AA 23-529
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool).
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Qualiy tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: This antibody blocks Glypican-4 mediated Wnt3a signaling in vitro at concentrations from 0.1 - 5.0 µg/mL.
RRID: AB_3720723 (BioLegend Cat. No. 386409) AB_3720723 (BioLegend Cat. No. 386410)
Structure: Cell surface heparan sulfate proteoglycan
Distribution: Cell membrane and nucleoplasm
Function: Enhances Wnt signaling by acting as R-spondin co-receptor
Interaction: PICK1, Insulin Receptor, Wnt3a, FGF2
Ligand/Receptor: Wnt3a
Cell Type: Neurons
Biology Area: Cell Biology, Cell Proliferation and Viability, Neurodegeneration, Signal Transduction
Molecular Family: APP/Aβ Degradation
Antigen References: Sakane H, et al. 2012. J Cell Sci. 125:449-60. Hagihara K, et al. 2000. Developmental Dynamics. 219:353-67. Watanabe K, et al. 1995. J Cell Biol. 130:1207-18. Ussar, S. et al. 2012. Diabetes. 61:2289-98.
Gene ID: 2239
UniProt: View information about Glypican-4 on UniProt.org
Clone: A21050B
Regulatory Status: RUO
Other Names: GPC4
Isotype: Mouse IgG2b, κ