Biolegend, 386511, APC/Cyanine7 anti-human AXL Antibody, 25tests

AXL, also known as ARK or UFO, is a widely expressed glycoprotein in the TAM (Tyro3/Dtk, AXL, and Mer) receptor tyrosine kinase family. AXL is highly expressed in solid cancers. It is also expressed on a small population of human dendritic cells that share characteristics of classical and plasmacytoid dendritic cells. AXL binds the vitamin K-dependent growth arrest specific 6 (Gas6) protein and induces receptor autophosphorylation and downstream signaling pathways that are related to cell proliferation, migration, or apoptosis. AXL is also involved in hematopoiesis, embryonic development, tumorigenesis, and regulation of testicular functions. Recent studies have also shown that AXL and Mer are both linked to Alzheimer's disease.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC/Cyanine7 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: Clone S21005C recognizes a different epitope compared to clone S21005B. Clone S21005C does not block GAS6 induced AKT and ERK phosphorylation in H1299 cells.
RRID: AB_3675112 (BioLegend Cat. No. 386511) AB_3675112 (BioLegend Cat. No. 386512)
Structure: Transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type domains, and one tyrosine kinase domain
Distribution: Highly expressed in solid cancers and also expressed on human blood plasmacytoid dendritic cells and myeloid dendritic cells
Function: Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that are related to cell proliferation, migration, or apoptosis. It is involved in hematopoiesis, embryonic development, tumorigenesis, and regulation of testicular functions. Recent studies have shown that AXL and Mer are both linked to Alzheimer's disease.
Ligand/Receptor: Gas6 and Protein S
Cell Type: Dendritic cells, NK cells
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cardiovascular Biology, Cell Death, Cell Proliferation and Viability, Immuno-Oncology, Signal Transduction
Molecular Family: Cytokine/Chemokine Receptors, Cytokines/Chemokines
Antigen References: Holland S, et al. 2005. Canc Res. 65:9294-303. Lee WP, et al. 2002. Oncogene. 21:329-36. Demarchi F, et al. 2001. J Biol Chem. 276:31738-44.
Gene ID: 558
UniProt: View information about AXL on UniProt.org
Clone: S21005C
Regulatory Status: RUO
Other Names: ARK, AXL receptor tyrosine kinase, AXL transforming sequence/gene, AXL oncogene, JTK11, Tyro7, tyrosine-protein kinase receptor UFO
Isotype: Mouse IgG1, κ