Biolegend, 387319, APC/Cyanine7 anti-human CD8b Antibody, 25tests

CD8b or CD8β antigen is also known as Lyt3, or Ly-3. It is a member of the immunoglobulin superfamily expressed on most thymocytes, and some mature T cell subsets. CD8β forms heterodimers with the CD8α chain (CD8a). CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells. CD8 participates in T cell activation through its association with the T cell receptor complex and protein tyrosine kinase LCK (p56 lck ).
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Immunogen: Human CD8α/CD8β-transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC/Cyanine7 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: Clone S21011A does not interfere with the binding of CD8α binding antibodies (SK1, HIT8a, RPA-T8).Clone S21011A shows comparable staining signals on 4% paraformaldehyde-fixed cells and unfixed cells.Based on in-house testing, clone S21011A clone reacts with an epitope that is exclusively present on human CD8b, whereas clone QA20A40 reacts with an epitope formed by a heterodimer of human CD8a and CD8b chains.
RRID: AB_3717009 (BioLegend Cat. No. 387319) AB_3717009 (BioLegend Cat. No. 387320)
Structure: Ig superfamily
Distribution: Subset of T Cells, most thymocytes
Function: TCR co-receptor
Interaction: CD8α
Ligand/Receptor: MHC Class 1 molecules
Cell Type: Lymphocytes, T cells, Thymocytes
Biology Area: Adaptive Immunity, Cancer Biomarkers, Cell Biology, Immunology
Molecular Family: CD Molecules, TCRs
Antigen References: Johnson P. 1987. Immunogenetics. 26:174-7. Norment AM & Littman DR, 1988. EMBO J. 7:3433-9.
Gene ID: 926
UniProt: View information about CD8beta on UniProt.org
Clone: S21011A
Regulatory Status: RUO
Other Names: Lyt3, Ly3, P37, LEU2, CD8B1, CD8b, CD8beta
Isotype: Mouse IgG1, κ