Biolegend, 387603, PE anti-human G-CSF Antibody, 25tests

Human G-CSF was cloned from a human squamous carcinoma cell line and is the key hematopoietic cytokine involved in the control of neutrophil production. Therefore, it is a critical regulator of innate immunity against bacterial infections. G-CSF mobilizes stem cells indirectly by downregulating the expression of CXCL12 on marrow osteoblasts and by releasing neutrophil and monocyte proteolytic enzymes, including neutrophil elastase, cathepsin G, and matrix metalloproteinase-9, which in turn degrades important HSC trafficking and adhesion molecules c-kit, VCAM-1, and CXCR4. In addition, G-CSF possesses immunosuppressive effects on monocytes/macrophages, dendritic cells, and T lymphocytes. The receptor for G-CSF is expressed not only in hematopoietic cells; it is also expressed in cardiomyocytes, skeletal muscle, and neurons. G-CSF influences mouse skeletal muscle development and regeneration by stimulating myoblast proliferation. In vitro , G-CSF displays strong antiapoptotic activity in neuronal cells.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant Human G-CSF
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Clones A20052D and A20052Q do not block each other.
RRID: AB_2941577 (BioLegend Cat. No. 387603) AB_2941577 (BioLegend Cat. No. 387604)
Structure: Heterodimer
Distribution: Macrophages, monocytes, neutrophils, fibroblasts, endothelial cells, epithelial cells, and bone marrow stromal cells
Function: G-CSF acts on myeloid progenitor cells to stimulate survival, proliferation, and neutrophilic maturation; on mature neutrophils, G-CSF enhances survival, superoxido anion and alkaline phosphatase production, arachidonic acid release, and antibody dependent cellular cytotoxicity.
Interaction: Myeloid progenitor cells
Ligand/Receptor: CD114 (G-CSFR)
Molecular Family: Cytokines/Chemokines
Antigen References: Nagata S, et al. 1986. Nature. 319:415-8. Kim HK, et al. 2006. Blood. 108:812-20. Donahue RE, et al. 2009. Blood. 114:2530-41. Shimoji K, et al. 2010. Cell Stem Cell. 6:227-37. Martins A, et al. 2010. IUBMB Life. 62:611-7. Hara M, et al. 2011. J Exp Med. 208:715-27.
Gene ID: 1441
UniProt: View information about G-CSF on UniProt.org
Clone: A20052Q
Regulatory Status: RUO
Other Names: Granulocyte-Colony Stimulating Factor, CSF3, CSF-β
Isotype: Rat IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.