Biolegend, 387703, PE anti-human CD66 (CEACAM4/6) Antibody, 25tests

CEACAM4, also known as CGM7, belongs to the immunoglobulin superfamily, specifically the carcinoembryonic antigen (CEA)-like subfamily. It is an orphan receptor that serves a phagocytic function in human granulocytes. CD66c, also known as CEACAM6, is one of the splice variants of CD66. It is expressed on endothelial, epithelial cells, neutrophils, monocytes, and myeloma and cancer cells, such as B-cell acute lymphoblastic lymphomas. CD66 expression level can be induced on T cells, B cells, and CD16-negative NK cells. Like the other CD66 splice variants, CD66c is involved in cell adhesion and growth, neutrophil activation, and signaling. It plays a role in hemophilic and heterophilic adhesion.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant protein, aa 35-320
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Clone W20030D binds both CEACAM4 and CEACAM6.
RRID: AB_2941578 (BioLegend Cat. No. 387703) AB_2941578 (BioLegend Cat. No. 387704)
Structure: Ig superfamily, CEA antigen group, GPI-linked glycoprotein
Distribution: Granulocytes, Monocytes, epithelial cells
Function: Cell adhesion, neutrophil activation, homophilic and heterophilic adhesion
Interaction: Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM5 and CEACAM8
Ligand/Receptor: CD66c, CD66a-e, CD62E and Galectins
Molecular Family: Adhesion Molecules
Antigen References: Delgado Tascón J, et al. 2015. J Leukoc Biol. 97(3):521-31. Duxbury MS, et al. 2004. Oncogene. 23:465–73. Oikawa S, et al. 1991. J Biol Chem. 266:7995–8001. Noworolska A, et al. 1986. Cancer Detect Prev. 9:365–71. Wu S, et al. 2021. Transl Onc. 14:101057.
Gene ID: 10894680
UniProt: View information about CD66 on UniProt.org
Clone: W20030D
Regulatory Status: RUO
Other Names: CEACAM4, CEACAM6
Isotype: Rat IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.