Biolegend, 387804, PE anti-human CD226 (DNAM-1) Antibody, 100tests

DNAM-1 (CD226) is a ~65 kD glycoprotein expressed on cell surface of T cells, NK cells, monocytes/macrophages, platelets and megakaryocytes and a subset of B cells and a member of the immunoglobulin (Ig)-superfamily containing 2 Ig-like domains of the V-set. The ligands for CD226 are the poliovirus receptor (CD155) and its family member nectin-2 (CD112), which are broadly expressed on epithelial, endothelial and neuronal cells. CD226 is physically associated with LFA-1 in NK cells and activated T cells, and involved in LFA-1-mediated signaling.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Human DNAM-1 Transfected Cells.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_3068124 (BioLegend Cat. No. 387803)
Structure: Ig domain containing adhesion molecule
Distribution: Peripheral blood T cells, NK cells, monocytes/macrophages, platelets and megakaryocytes and a subset of B cells
Function: Receptor involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-lymphocyte (CTL) and NK cell
Interaction: Interacts with LFA-1
Ligand/Receptor: PVR (CD155) and Nectin-2 (CD112)
Cell Type: B cells, Macrophages, Megakaryocytes, Monocytes, NK cells, Platelets, T cells
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: Shibuya A, et al. 1996. Immunity. 4:573-81. Tahara-Hanaoka S, et al. 2004. Int Immunol. 16:533-8. Pende D, et al. 2005. Mol. Immunol. 42:463-9.
Gene ID: 10666
UniProt: View information about CD226 on UniProt.org
Clone: W21097F
Regulatory Status: RUO
Other Names: DNAM-1, PTA1, TLiSA1
Isotype: Rat IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.