Biolegend, 388603, PE anti-human CD69 Antibody, 25tests

CD69 is a 27-33 kD type II transmembrane protein also known as activation inducer molecule (AIM), very early activation antigen (VEA), and MLR3. It is a member of the C-type lectin family, expressed as a disulfide-linked homodimer. Other members of this receptor family include NKG2, NKR-P1, CD94, and Ly49. CD69 is transiently expressed on activated leukocytes including T cells, thymocytes, B cells, NK cells, neutrophils, and eosinophils. CD69 is constitutively expressed by a subset of medullary mature thymocytes, platelets, mantle B cells, and certain CD4+ T cells in germinal centers of normal lymph nodes. CD69 is involved in early events of lymphocyte, monocyte, and platelet activation, and has a functional role in redirected lysis mediated by activated NK cells.
25tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Chimpanzee, Cynomolgus, Pig
Antibody Type: Monoclonal
Host Species: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_3083391 (BioLegend Cat. No. 388603) AB_3083391 (BioLegend Cat. No. 388604)
Structure: C-type lectin, type II glycoprotein, 28/32 kD
Distribution: Activated T cells, B cells, NK cells, granulocytes, thymocytes, platelets, Langerhans cells
Function: Lymphocyte, monocyte, and platelet activation, NK cell killing
Cell Type: B cells, Lymphocytes, NK cells, Platelets, T cells, Thymocytes
Biology Area: Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: Testi R, et al. 1994. Immunol Today. 15:479-83.
Gene ID: NA
UniProt: View information about CD69 on UniProt.org
Clone: W19310K
Regulatory Status: RUO
Other Names: Very Early Activation Antigen (VEA), Activation Inducer Molecule (AIM)
Isotype: Rat IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.