Biolegend, 388705, FITC anti-human CD8a Antibody, 25tests

CD8α, also known as T8 or Leu2, is a 32-34 kD type 1 transmembrane glycoprotein part of the immunoglobulin superfamily. CD8α is expressed on cytotoxic T cells, NK cells, as well as a majority of thymocytes. CD8α can form a homodimer (CD8α/α) or heterodimer (CD8α/β) with CD8β. CD8α acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation, and has been shown to play a role in thymic differentiation. Two domains in CD8α are important for function: the extracellular Ig superfamily domain binds the α3 domain of MHC Class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human CD8α-transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Clone S21010A can completely block the binding of clone HIT8a on target cells and can be blocked by HIT8a. Clone S21010A does not block the binding of clone SK1, clone RPA-T8, and clone S21010F. Clone S21010A does not interfere with the binding of anti-human CD8β antibodies S21011A or QA20A40. Clone S21010A shows comparable staining signals on 4% paraformaldehyde-fixed cells and unfixed cells.
RRID: AB_3662286 (BioLegend Cat. No. 388705) AB_3662286 (BioLegend Cat. No. 388706)
Structure: Ig superfamily
Distribution: Subset of T cells, most thymocytes, NK cells
Function: TCR co-receptor
Interaction: CD8β
Ligand/Receptor: MHC Class I molecules
Cell Type: NK cells, T cells, Thymocytes
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego. Leahy, D.J, 1995. FASEB J. 9:17-25.
Gene ID: 925
UniProt: View information about CD8alpha on UniProt.org
Clone: S21010A
Regulatory Status: RUO
Other Names: T8, Leu2, CD8 Antigen
Isotype: Mouse IgG1, κ