Biolegend, 388816, PE/Cyanine7 anti-human CD8a Antibody, 100tests

CD8α, also known as T8 or Leu2, is a 32-34 kD type 1 transmembrane glycoprotein part of the immunoglobulin superfamily. CD8α is expressed on cytotoxic T cells, NK Cells, as well as a majority off thymocytes. CD8α can form a homodimer (CD8α/α) or heterodimer (CD8α/β) with CD8β. CD8α acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation, and has been shown to play a role in thymic differentiation. Two domains in CD8α are important for function: the extracellular Ig superfamily domain binds the α3 domain of MHC Class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human CD8α-transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Clone S21010F can completely block the binding of clone HIT8a, clone RPA-T8, and clone SK1 on target cells and can be blocked by HIT8a and SK1. Clone S21010F does not interfere with the binding of anti-human CD8β antibodies S21011A or QA20A40. Clone S21010F shows comparable staining signals on 4% paraformaldehyde-fixed cells and unfixed cells.
RRID: AB_3683438 (BioLegend Cat. No. 388815) AB_3683438 (BioLegend Cat. No. 388816)
Structure: Ig superfamily
Distribution: Subset of T Cells, most thymocytes, NK cells
Function: TCR co-receptor
Interaction: CD8β
Ligand/Receptor: MHC Class I molecules
Cell Type: NK cells, T cells, Thymocytes
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego. Leahy, D.J, 1995. FASEB J. 9:17-25.
Gene ID: 925
UniProt: View information about CD8alpha on UniProt.org
Clone: S21010F
Regulatory Status: RUO
Other Names: T8, Leu2, CD8 Antigen
Isotype: Mouse IgG2b, κ