Biolegend, 389204, PE anti-human CD252 (OX40L) Antibody, 100tests

OX40 ligand (OX40L), also known as gp34, CD252, CD134L or TNFSF4, is a 35 kD type II transmembrane protein which belongs to TNF superfamily. The OX40 ligand is expressed on activated B cells, activated endothelial cells, mature dendritic cells, activated CTLs, activated mast cells, and airway smooth muscle cells. OX40 ligand interacts with OX40 antigen (CD134) expressed predominantly on activated T cells to regulate T cell proliferation and differentiation, and to enhance immunoglobulin secretion by activated B cells.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant human OX40L protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_3106062 (BioLegend Cat. No. 389203) AB_3106062 (BioLegend Cat. No. 389204)
Structure: NGF/TNF superfamily, 35 kD
Distribution: Activated B cells, activated endothelial cells, mature dendritic cells, airway smooth muscle cells
Function: T-cell interaction, costimulation
Ligand/Receptor: OX40 (CD134)
Cell Type: B cells, Dendritic cells, Endothelial cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: Imura A, et al. 1996. J Exp Med. 183:2185-95. Ohshima Y, et al. 1997. J Immunol. 159:3838-48. Ito T, et al. 2005. J Exp Med. 202:1213-23. Ito T, et al. 2006. Proc Natl Acad.Sci. USA. 103:13138.
Gene ID: 7292
UniProt: View information about CD252 on UniProt.org
Clone: W20047C
Regulatory Status: RUO
Other Names: Gp34, OX40L, CD134L, CD252, TNFSF4
Isotype: Rat IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.