Biolegend, 389510, APC/Fire™ 750 anti-human CD158e1 (KIR3DL1, NKB1) Antibody, 100tests

CD158 molecules, also known as KIRs (killer cell immunoglobulin-like receptors), are a family of transmembrane proteins with either two (KIR2D) or three (KIR3D) Ig-like extracellular domains. Some KIRs with long cytoplasmic domains contain ITIMs and possess inhibitory functions and others with short cytoplasmic region lack ITIM and have activation functions. Fifteen polymorphic KIR genes have been reported in humans. CD158e is a 50 kD three extracellular Ig domain glycoprotein that displays a long cytoplasmic tail with two ITIM motifs. It is expressed primarily on a subset of NK cells and a subset of T cells. KIR3DL1 has been reported to bind to a number of different MHC class molecules that contain the HLA Bw4 epitope and CD8aa.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: KIR3DL1-transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.
Excitation Laser: Red Laser (633 nm)
Application Notes: Clone S22024B does not block the binding of clone DX9 on target cells. Based on in-house testing on different KIR-expressing transfectants, clone S22024B does not interact with other KIR protein family members, including CD158e2.
RRID: AB_3717162 (BioLegend Cat. No. 389509) AB_3717162 (BioLegend Cat. No. 389510)
Structure: 50 kD three Ig-like extracellular domain glycoprotein
Distribution: Subset of NK cells, subset of T Cells
Function: NK Cell regulation
Ligand/Receptor: MHC Class 1 molecules
Cell Type: Lymphocytes, NK cells, T cells
Biology Area: Cell Biology, Immunology, Innate Immunity
Molecular Family: CD Molecules
Antigen References: Lanier LL, 2005. Annu Rev Immunol. 23:225-74. Middleton D, et al. 2010. Immunol. 123:8. Khakoo SI, et al. 2002. J Exp Med. 196:911-21.
Gene ID: 3811
UniProt: View information about CD158e1 on UniProt.org
Clone: S22024B
Regulatory Status: RUO
Other Names: CD158e, Killer Cell Immunoglobulin-Like Receptor 3DL1, NKAT3, NKB1
Isotype: Mouse IgG1, κ