Biolegend, 389807, PE anti-human CD90 (Thy1) Antibody, 25tests

CD90, also known as Thy-1, is a 25-35 kD GPI-anchored protein. It belongs to the Ig superfamily. Human CD90 is expressed on neuronal cells, a subset of CD34+ cells, a subset of fetal liver cells and fetal thymocytes, fibroblasts, activated endothelial cells, and some leukemia cell lines. CD34+CD90+ cells are primitive hematopoietic stem cells. It has been reported that Thy-1 binds with β2 and β3 integrins and plays bimodal roles in the regulation of cell adhesion and neurite outgrowth, and inhibits hematopoietic stem cells proliferation and differentiation.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human CD90-transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool).
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Clone S22015B is able to completely block the binding of clone 5E10 on target cells. Clone S22015B does not block the binding of clone S22015F on target cells.
RRID: AB_3717199 (BioLegend Cat. No. 389807) AB_3717199 (BioLegend Cat. No. 389808)
Structure: 25-35 kD glycoprotein, Ig superfamily
Distribution: Subset of CD34+ hematopoietic stem cells, subset of fetal thymocytes, subset of fetal liver cells, fibroblast, activated endothelial cells, neurons, and some leukemia cell lines
Function: Cell adhesion
Cell Type: Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors, Leukemia, Neurons, Thymocytes
Biology Area: Cell Adhesion, Neurodegeneration, Neuroscience, Stem Cells
Molecular Family: CD Molecules
Antigen References: McKenzie JL, et al. 1981. J Immunol. 126:843-50. Avalos AM, et al. 2002. Biol Res. 35:231-8. Wetzel A, et al. 2004. J Immunol. 172:3850-9.
Gene ID: 7070
UniProt: View information about CD90 on UniProt.org
Clone: S22015B
Regulatory Status: RUO
Other Names: Thy-1, Thy1
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.