Biolegend, 390604, Cell-Vive™ GMP Ultra-LEAF™ Purified anti-HU CD45 RecAb SF, 1mg

CD45 is a 180-240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. BioLegend Cell-Vive™ GMP Ultra-LEAF™ antibodies are manufactured and tested in accordance with USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Ph. Eur. Chapter 5.2.12 in a dedicated GMP facility compliant with ISO 13485:2016. Specifications and processes include
1mg
Reactivity: Human
Antibody Type: Recombinant
Host Species: Mouse
Formulation: 0.1 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Endotoxin Level: Less than 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test
Preparation: GMP Ultra-LEAF™ (Low Endotoxin, Azide-Free) Recombinant Serum Free antibody purified by affinity chromatography
Concentration: 1 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.50 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections9, inhibition of CD45 functions4, immunofluorescence11, Western blotting3, and spatial biology (IBEX)16,17.
Application References(PubMed link indicates BioLegend citation): Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. Kishihara K, et al. 1993. Cell. 74:143. Esser M, et al. 2001. J. Virol. 75:6173. (WB) Yamada T, et al. 2002. J. Biol. Chem. 277:28830. Nagano M, et al. 2007. Blood. 110:151. Jiang Q, et al. 2008. Blood. 112:2858. PubMed Morozov A, et al. 2010. Clin Cancer Res. 16:5630. PubMed Yoshino N, et al. 2000. Exp. Anim. 49:97. (FC) Friedman T, et al. 1999. J. Immunol. 162:5256. (IHC) Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed Rees LE, et al. 2003. Clin. Exp. Immunol. 134:497. (IF) Lee J, et al. 2015. J Exp Med. 212:385. PubMed
Disclaimer: BioLegend Cell-Vive™ GMP Ultra-LEAF™ antibodies are for research use only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.
Structure: Tyrosine phosphatases, type I transmembrane protein, 180-240 kD (multiple isoforms)
Distribution: Hematopoietic cells, not expressed in circulating erythrocytes or platelets
Function: TCR and BCR mediated activation
Interaction: Hematopoietic stem and progenitors, Mesenchymal Stem Cells
Ligand/Receptor: Galectin-1, CD2, CD3, CD4
Gene ID: 5788
UniProt: View information about CD45 on UniProt.org
Clone: HI30.Rec
Other Names: LCA, T200
Isotype: Mouse IgG1, κ