Biolegend, 391705, APC anti-human Folate Receptor β (FR-β) Antibody, 25tests

Folate receptors beta (FR-β) is a member of a family of folate binding receptors that have diverse structural identities but mediate transport of folates into cells. FR-β is a GPI-anchored protein with high affinity for folic acid. It is postulated that this receptor function as folate scavengers when folate supply is low or rapid cell growth requires elevated uptake of folate for methylation reactions including DNA biosynthesis. Although this protein was originally thought to be specific to placenta, it can also exist in other tissues, and it may play a role in the transport of methotrexate in synovial macrophages in rheumatoid arthritis patients.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human Folate beta transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Application References(PubMed link indicates BioLegend citation): Nagayoshi R, et al. 2005. Arthritis Rheum. 9:2666
Product Citations: Rodriguez-García A, et al. 2021. Nat Commun. 12:877. PubMed Appios A, et al. 2021. Bio Protoc. 11:e4044. PubMed Mulder K, et al. 2021. Immunity. 54(8):1883-1900.e5. PubMed
RRID: AB_2721302 (BioLegend Cat. No. 391705) AB_2721303 (BioLegend Cat. No. 391706)
Structure: 30 kD, seven disulfide bonds
Distribution: Placenta, monocytes, tissue resident macrophages
Function: Transport of folate into cells
Ligand/Receptor: Folic Acid
Cell Type: Macrophages, Monocytes
Biology Area: Immunology
Antigen References: Elwood PC. 1989. J Biol Chem. 264:14893 Shen F, et al. 1994. Biochemistry. 33:1209 Yi YS. 2016. Immune Netw. 16:337
Gene ID: 2350
UniProt: View information about Folate Receptor beta on UniProt.org
Clone: 94b/FOLR2
Regulatory Status: RUO
Other Names: FR-BETA
Isotype: Mouse IgG1, κ