Biolegend, 391906, APC anti-human GPR56 Antibody, 100tests

GPR56, also known as TM7XN1, is an orphan G-protein-coupled receptor (GPCR) containing seven transmembrane domains and a mucin-like domain in the N-terminal region. The total length of the human GPR56 gene is approximately 15 kb, and it consists of 13 exons and encodes 693 amino acids.  GPR56 is expressed in a wide range of tissues, such as brain, thyroid gland, and heart. Mutations in human GPR56 gene were found to cause a specific brain malformation called bilateral frontoparietal polymicrogyria. Recently, GPR56 was found specifically expressed on cytotoxic NK and T lymphocytes, including CD8 + , CD4 + , and γδ T cells, which might affect the migratory properties of these cells. GPR56 was also found significantly downregulated in some cancer cells, suggesting that it may play a role in growth suppression and cancer metastasis.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human GPR56 extracellular domain, complete Freund’s adjuvant, incomplete Freund’s adjuvant
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: blocking the cell migration (1).
Application References(PubMed link indicates BioLegend citation): Ohta S, et al. 2015. Biol. Pharm. Bull. 38:594.
RRID: AB_2716186 (BioLegend Cat. No. 391905) AB_2716187 (BioLegend Cat. No. 391906)
Structure: Contains seven transmembrane domains and a mucin-like domain in the N-terminal region.
Distribution: T cells, NK cells, wide range of tissues such as brain, thyroid gland, and heart.
Function: May be involved in immune cell migration, cancer cell growth metastasis, and neuron development.
Cell Type: NK cells, T cells
Antigen References: 1. Peng YM, et al. 2011. J. Leuko. Biol. 90:735. 2. Shashidhar S, et al. 2005. Oncogene 24:1673. 3. Piao X, et al. 2004. Science 303:2033. 4. Liu M, et al. 1999. Genomics 55:296.
Gene ID: 9289
UniProt: View information about GPR56 on UniProt.org
Clone: 4C3
Regulatory Status: RUO
Other Names: TM7XN1, ADGRG1
Isotype: Mouse IgG1, κ