Biolegend, 392116, Alexa Fluor® 647 anti-human P2RY12 Antibody, 100tests

P2RY12 is a receptor for ADP and ATP coupled to G-proteins that inhibit the adenylyl cyclase second messenger system and is not activated by UDP and UTP. P2RY12 is required for normal platelet aggregation and blood coagulation, and is a target to inhibit of platelet aggregation in the treatment of thromboembolisms and other clotting disorders. P2RY12 is expressed on platelets, lung, appendix, pituitary and adrenal glands; this molecule is a very useful marker to identify microglial cells in the brain.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human P2RY12-transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: View the video of our collaborator discussing the importance of highly specific monoclonal antibodies for P2RY12 in Microglia research.
RRID: AB_3068171 (BioLegend Cat. No. 392115) AB_3068171 (BioLegend Cat. No. 392116)
Distribution: Platelets, microglia
Function: Inhibit the adenylyl cyclase second messenger system
Ligand/Receptor: ADP, ATP
Cell Type: Microglia, Platelets
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family: GPCR, Purinergic Receptors
Antigen References: 1. Lecchi A, et al. 2015. Blood. 125:1006. 2. Zhang K, et al. 2014. Nature. 509:115. 3. Zhang J, et al. 2014. Nature. 509:119. 4. Cornelissen I, et al. 2010. Proc. Natl. Acad. Sci. USA. 107:18605. 5. Paruchuri S, et al. 2009. J. Exp. Med. 206:2543.
Gene ID: 64805
UniProt: View information about P2RY12 on UniProt.org
Clone: S16001E
Regulatory Status: RUO
Other Names: ADPG-R, BDPLT8, HORK3, P2T(AC), P2Y(12)R, P2Y(AC), P2Y(ADP), P2Y(cyc), P2Y12 platelet ADP receptor, purinergic receptor P2RY12, purinergic receptor P2Y, G-protein coupled
Isotype: Mouse IgG2a, κ