Biolegend, 392509, PerCP anti-human CD19 Antibody, 25tests

CD19 is a 95 kD type I transmembrane glycoprotein also known as B4. It is a member of the immunoglobulin superfamily expressed on B-cells (from pro-B to blastoid B cells, absent on plasma cells) and follicular dendritic cells. CD19 is involved in B cell development, activation, and differentiation. CD19 forms a complex with CD21 (CR2) and CD81 (TAPA-1), and functions as a BCR co-receptor.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human Chronic Lymphocytic Leukemia (CLL) cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Clone 4G7 partially blocks anti-human CD19 clones HIB19 and SJ25C1 staining based on in-house testing
Application References(PubMed link indicates BioLegend citation): Cabezudo E, et al. 1999. Haematologica. 84:413-8 Van Zelm MC et al. 2010. J Clin Invest. 120:1265-74
Product Citations: Vielle NJ, et al. 2018. Sci Rep. 8:5440. PubMed Xue D, et al. 2016. Front Immunol. 558:7. PubMed Su W, et al. 2022. Front Immunol. 13:952338. PubMed
RRID: AB_2750100 (BioLegend Cat. No. 392509) AB_2810585 (BioLegend Cat. No. 392510)
Structure: Ig superfamily, type I transmembrane glycoprotein, 95 kD
Distribution: Normal and malignant B lymphocytes
Function: B cell activation and differentiation
Ligand/Receptor: Forms complex with CD21 (CR2) and CD81 (TAPA-1), BCR coreceptor
Cell Type: B cells, Lymphocytes
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: Tedder T, et al. 1994. Immunol. Today 15:437. Bradbury L, et al. 1993. J. Immunol. 151:2915.
Gene ID: 930
UniProt: View information about CD19 on UniProt.org
Clone: 4G7
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: B4, CVID3
Isotype: Mouse IgG1, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.