Biolegend, 393509, FITC anti-human CD244 (2B4) Antibody, 25tests

CD244, known as 2B4, is a 38 kD type I transmembrane protein. It is a member of the CD2 subset of the immunoglobulin superfamily (IgSF) molecules. CD244 is expressed on NK cells, a subset of T cells (including most CD8 + T cells and γ/δ T cells), monocytes, basophils, and eosinophils. CD48 is the ligand of CD244. It has been reported that ligation of human CD244 results in enhanced NK cell cytotoxicity and cytokine production. Recent studies have shown that human CD244, like murine CD244, has both activating and inhibitory functions, which are dependent on the density of surface 2B4 expression, degree of ligation, and the level of the adaptor molecule SAP expression.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human NK cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: redirected killing assays (rADCC) in NK cells1.
Application References(PubMed link indicates BioLegend citation): Nakajima H, et al. 2000. Eur J Immunol. 30:3309. (Cytotoxicity, IP) Tassi I, Colonna M. 2005. J. Immunol. 7996-8002. (IP)
RRID: AB_2734467 (BioLegend Cat. No. 393509) AB_2734468 (BioLegend Cat. No. 393510)
Structure: 38 kD type I transmembrane protein, CD2 family member, Ig superfamily
Distribution: NK, T subset, monocytes, basophils, and eosinophils
Function: Activation and inhibition of NK cell function
Ligand/Receptor: CD48
Cell Type: Basophils, Eosinophils, Monocytes, NK cells
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: Lee KM, et al. 2003. J. Immunol. 170:4881. Chlewicki LK, et al. 2008. J. Immunol. 180:8159. Messmer B, et al. 2006. J. Immunol. 176:4646. Boles KS, et al. 2001. Immunol. Rev. 181:234.
Gene ID: 51744
UniProt: View information about CD244 on UniProt.org
Clone: 2-69
Regulatory Status: RUO
Other Names: 2B4, NAIL, SLAMF4
Isotype: Mouse IgG2a, κ