Biolegend, 394504, PE anti-human CD285 (TLR5) Antibody, 100tests

CD285, also known as TLR5, is a 98 kD pattern recognition receptor that binds to bacterial flagellin. It is expressed on dendritic cells, classical monocytes and mucosal epithelial cells and is involved in activation of TLR5 induced NF-κB mediated activation of inflammatory-related target genes. Mutations in the TLR5 gene have been linked with susceptibility to systemic lupus erythematosus and Legionnaire’s disease.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human TLR5 transfectants
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations: González-Stegmaier R, et al. 2023. Molecules. 28:. PubMed
RRID: AB_2750013 (BioLegend Cat. No. 394503) AB_2750014 (BioLegend Cat. No. 394504)
Structure: Type 1 transmembrane protein, 98 kD
Distribution: Neutrophils, classical monocytes, dendritic cells, mucosal epithelium
Function: Interacts with MyD88 and TRAF6 leading to NF-κB activation and production of cytokines.
Ligand/Receptor: flagellin
Cell Type: Dendritic cells, Epithelial cells, Monocytes, Neutrophils
Biology Area: Immunology
Molecular Family: CD Molecules, Toll Like Receptors
Antigen References: Hayashi F, et al. 2001. Nature. 410:1099 Gewirtz AT, et al. 2001. J Immunol. 167:1882 Smith KD, et al. 2002. Curr Top Microbiol Immunol. 270:93 Bambou JC, et al. 2004. J Biol Chem. 279:42984 Sebastiani G, et al. 2000. Genomics. 64:230 Muzio M, et al. 2000. Biochem Soc Trans. 28:563
Gene ID: 7100
UniProt: View information about CD285 on UniProt.org
Clone: S16021I
Regulatory Status: RUO
Other Names: SLE1, TIL3, SLEB1, MELIOS, CD285
Isotype: Mouse IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.