Biolegend, 394805, Brilliant Violet 421™ anti-human CD289 (TLR9) Antibody, 25tests

TLR9 is a member of the toll-like receptor family that aids in recognition of pathogen associated molecular patterns (PAMPs). TLR9 recognizes unmethylated CpG sequences in DNA (ie. intracellular bacteria and DNA viruses). TLR9 signals through the MyD88 pathway leading to inflammatory cytokine production, especially production of type I interferons by pDCs.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: CD289 (TLR9)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC, FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: S16013D clone can be used for both surface and intracellular detection of TLR9. ICFC compatible with both the intracellular flow cytometric staining and True-Nuclear™ transcription buffer set. Does not work for WB (tested on Daudi cell line).
RRID: AB_2801039 (BioLegend Cat. No. 394805) AB_2801040 (BioLegend Cat. No. 394806)
Distribution: TLR9 is expressed by B cells and plasmacytoid dendritic cells (pDC).
Ligand/Receptor: TLR9 recognizes unmethylated CpG sequences in DNA.
Cell Type: B cells, Dendritic cells
Biology Area: Bacterial proteins and Toxins, Immuno-Oncology, Immunology, Innate Immunity
Molecular Family: CD Molecules, Innate Immune Signaling, Toll Like Receptors
Antigen References: Hornung V, et al. 2002. J. Immunol. 168:4531. Eaton-Bassiri A, et al. 2004. Infect. Immun. 72:7202. Krieg, A. 2007. J. Clin. Invest. 117:1184.
Gene ID: 54106
UniProt: View information about CD289 on UniProt.org
Clone: S16013D
Regulatory Status: RUO
Other Names: CD289, TLR9, Toll-like receptor 9
Isotype: Rat IgG2a, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.