Biolegend, 395509, Brilliant Violet 421™ anti-human CD288 (TLR8) Antibody, 25tests

Toll-like receptor (TLR) 8, also known as CD288, is a 83 kDa, type 1 transmembrane protein, member of the TLR receptor family. TLR8 is expressed intracellulary in monocytes, macrophages and myeloid dendritic cells, and plays an important role in the innate immune response by binding single-stranded RNA (ssRNA). TLR8 triggers anti-viral reponses promoting the secretion of proinflammatory cytokines such as IL-12 and TNF.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human TLR8-transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
RRID: AB_2876712 (BioLegend Cat. No. 395509) AB_2876712 (BioLegend Cat. No. 395510)
Structure: 83 kD, Type 1 transmembrane protein
Distribution: Endosomal compartments of monocytes, macrophages, myeloid dendritic cells, and neutrophils
Function: Binds single-stranded RNA
Interaction: MYD88, TRAF6, IRAK4
Ligand/Receptor: Single-stranded GU-rich RNA
Cell Type: Dendritic cells, Macrophages, Monocytes, Neutrophils
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, Toll Like Receptors
Antigen References: Lood C, et al. 2017. J Exp Med. 214:2103. Prinz N, et al. 2011. Blood. 118:2322. Gringhuis SI, et al. 2010. Nat Immunol. 11419. Cros J, et al. 2010. Immunity. 33:375. Peng G, et al. 2005. Science. 309:1380.
Gene ID: 51311
UniProt: View information about CD288 on UniProt.org
Clone: S16018A
Regulatory Status: RUO
Other Names: CD288, Toll-like receptor 8
Isotype: Mouse IgG2a, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.