Biolegend, 395603, PE anti-human CD7 Antibody, 25tests

CD7 is a 40 kD type I transmembrane glycoprotein also known as gp40. It is a member of the immunoglobulin superfamily found on T cells, NK cells, thymocytes, hematopoietic progenitors, and monocytes (weakly). CD7 is also expressed on acute lymphocytic leukemia (ALL) and some acute myeloid leukemia (AML) cells. CD7 crosslinking induces a calcium flux in T lymphocytes, presumably as a result of cytoplasmic domain association with PI3-kinase. CD7 costimulation can induce cytokine secretion and modulate cellular adhesion.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: T-ALL blast cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations: Lee SHR, et al. 2023. Nat Med. 29:170. PubMed Heckler M, et al. 2021. Cancer Discov. Online ahead of prin. PubMed
RRID: AB_2820048 (BioLegend Cat. No. 395603) AB_2820049 (BioLegend Cat. No. 395604)
Structure: Ig superfamily, type I transmembrane glycoprotein, 40 kD
Distribution: T cells, NK cells, thymocytes, hematopoietic progenitors, myeloid leukemic cell subsets
Function: T cell activation
Ligand/Receptor: Cytoplasmic domain associates with P13-kinase
Cell Type: Hematopoietic stem and progenitors, Leukemia, NK cells, T cells, Thymocytes
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego. Stillwell R and Bierer BE. 2001. Immunol Res. 24:31-52. Rabinowich H, et al. 1994. J Immunol. 152:517-26.
Gene ID: 924
UniProt: View information about CD7 on UniProt.org
Clone: 4H9/CD7
Regulatory Status: RUO
Other Names: gp40
Isotype: Mouse IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.