Biolegend, 395906, APC anti-human IRF3 Antibody, 100tests

IRF-3 is a roughly 50 kD member of the interferon regulatory transcription factor (IRF) family. Along with other members of the IRF family IRF-3 plays a key role in the innate immune response to viral infection. It is located ubiquitously in an inactivated form in the cytoplasm of many immune cells. When phosphorylated it forms a complex with CREBBP to upregulate many immune responses integral to innate immune function.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human IRF-3 protein (entire protein)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application References(PubMed link indicates BioLegend citation): Rustagi A, et al. 2012. Methods. 59(2):225-32
RRID: AB_2888751 (BioLegend Cat. No. 395905) AB_2888751 (BioLegend Cat. No. 395906)
Structure: Human IRF-3 has 427 amino acids with predicted Mw 47.2 kD and observed Mw 52 kD. Contains an IRF tryptophan pentad repeat responsible for DNA binding. Mouse Irf-3 has 419 amino acids with predicted MW 46.9 kD and observed Mw 45 kD.
Distribution: Inactive IRF-3 continuously transits between the cytoplasm and nucleus. Serine/threonine phosphorylation or IRF-3 results in the structural changes which allow IRF-3 activation. The activated IRF3 translocates to the nucleus, where it forms a complex with CREB binding protein and activates the transcription of interferons, interferon stimulated genes, and other antiviral response genes.
Function: CREB binding protein, IRF-7, TICAM-1, TICAM-2, non-canonical IKB kinases TBK1 and IKKε.
Biology Area: Cell Biology, Immunology, Innate Immunity, Signal Transduction, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: Pattwell SS, et al. 2017. Trends Mol Med. (9):773-776 Zhang ZX, et al. 2017. Leuk Lymphoma. 58(3): 646-654 Zhao B, et al. 2016. Proc Natl Acad Sci USA. 113(24)E3403-12
Gene ID: 3661
UniProt: View information about IRF3 on UniProt.org
Clone: AR1
Regulatory Status: RUO
Other Names: IRF3, IRF-3, interferon regulatory factor 3
Isotype: Mouse IgG1, κ