Biolegend, 396103, APC anti-human/mouse/rat MR1 Antibody, 25tests

MHC-related protein 1, also known as MR1, is a nonclassical MHC class I molecule highly conserved in mammals, particularly in its α1/α2 domains. MR1 is required for the development and expansion of the mucosal-associated invariant T (MAIT) cells. The transcripts for MR1 are ubiquitously expressed, but its expression on the cell surface seems to be ligand-dependent. MR1 is involved in the presentation of vitamin-derived compounds from bacterial biosynthetic pathways.
25tests
Verified Reactivity: Human, Mouse, Rat
Reported Reactivity: Cow
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Chimeric mMR1/L molecule (mMR1 [alpha1-alpha2 domain]/Ld [alpha3 cytoplasmic tail]) soluble, recombinant protein secreted by insect cells was used to immunize MR1 KO mice
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Application Notes: This antibody is noted to cross-react to over-expressed mouse, rat, bovine (cow) and human MR11. This clone does not cross-block clone 26.5 based on in-house testing.
Application References(PubMed link indicates BioLegend citation): Chua WJ, et al. 2011. J Immunol. 186:4744-50
RRID: AB_2814363 (BioLegend Cat. No. 396103) AB_2814364 (BioLegend Cat. No. 396104)
Structure: Member of the MHC class I family, 1 Ig-like C1-type domain, forms heterodimer with B-2 Microglobulin.
Distribution: MR1 transcripts are ubiquitously expressed, but its expression in the cell surface seems to be ligand-dependent.
Function: Antigen presentation, required for development and expansion of the mucosal-associated invariant T (MAIT) cells.
Ligand/Receptor: α-mannosyl ceramide derivatives, 6-formyl pterin, derivatives from bacterial riboflavin biosynthetic pathway).
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: Gold MC and Lewinsohn DM. 2013. Nat Rev Microbiol. 11:14. Young MH and Gapin L. 2013. Cell Res. 23:460. Gold MC, et al. 2013. Mucosal Immunol. 6:35. Meierovics A, et al. 2013. Proc Natl Acad Sci USA. 110:E3119.
Gene ID: 31401506425119
UniProt: View information about MR1 on UniProt.org
Clone: 8F2.F9
Regulatory Status: RUO
Other Names: Major Histocompatibility Complex Class I-related, HLALS
Isotype: Mouse IgG1, κ