Biolegend, 397120, Brilliant Violet 421™ anti-human CD38 Antibody, 100tests

CD38 is a 45 kD type II transmembrane glycoprotein also known as T10. It is an ADP-ribosyl hydrolase expressed at variable levels on hematopoietic cells and in some non-hematopoietic tissues (such as brain, muscles, and kidney). In humans, it is expressed at high levels on plasma cells and activated T and B cells. By functioning as both a cyclase and a hydrolase, CD38 mediates lymphocyte activation, adhesion, and the metabolism of cADPR and NAADP. CD31 is the ligand of CD38.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human CD38 transfectants
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: S17015A does not cross-block binding of clones HIT2, HB-7 or S17015F also raised against human CD38 based on in-house testing.
Product Citations: Meng L, et al. 2024. Front Immunol. 1440819:15. PubMed
RRID: AB_2904420 (BioLegend Cat. No. 397119) AB_2904420 (BioLegend Cat. No. 397120)
Structure: ADP-ribosyl cyclase, ectoenzyme, type II glycoprotein, 45 kD
Distribution: T cells, B cells, NK, myeloid, plasma, and dendritic cells
Function: Ecto-ADP-ribosyl cyclase, calcium signaling, cell activation
Ligand/Receptor: CD31, hyaluronic acid
Cell Type: B cells, Dendritic cells, NK cells, Plasma cells, T cells
Biology Area: Cell Adhesion, Cell Biology, Immunology
Antigen References: Ferrero E, et al. 1999. J Leukoc Biol. 65:151. Lund F, et al. 1995. Immunol Today. 16:469.
Gene ID: 952
UniProt: View information about CD38 on UniProt.org
Clone: S17015A
Regulatory Status: RUO
Other Names: HB7, T10, ADP-ribosyl cyclase
Isotype: Mouse IgG1, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.