Biolegend, 397907, FITC anti-human CD133 Antibody, 25tests

CD133, also known as Prominin-1 and AC133 antigen, is a 120 kD pentaspan glycoprotein with 5 transmembrane domains. CD133 was initially described as a surface antigen specific for human hematopoietic stem cells and as a marker for murine neuroepithelial cells and some embryonic epithelia. Later on, CD133 was found on other stem cells, including endothelial progenitor cells, glioblastomas, neuronal, and glial stem cells. In addition to stem cells for normal tissue, CD133 was found on cancer cells, such as some leukemia cells and brain tumor cells. Although the biological function of CD133 is not completely understood, CD133 has been extensively used as a stem cell marker for normal and cancerous tissues.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: WERI-RB-1 human retinoblastoma cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Application Notes: W6B3C1 antibody does not compete against AC133, 293C2, clone 7, S16015F, S16016B, or S16016E antibody binding to target.
Application References(PubMed link indicates BioLegend citation): Hermansen SK, et al. 2011. J. Histochem Cytochem. 59(4): 391. Rappa G, et al. 2008. Stem Cells. 26(12): 3008. Tardito S, et al. 2015. Nat Cell Biol. 17(12): 1556.
RRID: AB_3068114 (BioLegend Cat. No. 397907) AB_3068114 (BioLegend Cat. No. 397908)
Structure: 120 kD pentaspan transmembrance glycoprotein with 5 transmembrane domains.
Distribution: Hematopoietic stem cells and progenitor cells, fetal liver cells, tissue specific stem cells or progenitor cells such as renal and prostate, a variety of tumor cells.
Function: May play a role in cell differentiation, proliferation, and apoptosis.
Interaction: NAT8; NAT8B; CDHR1
Cell Type: Hematopoietic stem and progenitors, Leukemia, Leukocytes
Biology Area: Cancer Biomarkers, Cell Biology, Immuno-Oncology
Molecular Family: CD Molecules
Antigen References: Yin AH, et al. 1997. Blood. 90:5002. Miraglia S, et al. 1997. Blood. 90:5013. Bühring HJ, et al. 1999. Ann. NY Acad. Sci. 872:25.
Gene ID: 8842
UniProt: View information about CD133 on UniProt.org
Clone: W6B3C1
Regulatory Status: RUO
Other Names: Prominin-1, PROM1, AC133
Isotype: Mouse IgG1, κ