Biolegend, 398004, PE F(ab')2 Goat anti-human IgG Fcγ Antibody, 100microl

IgG Fc is a homodimer that is composed of the constant region of the two heavy chains that form the IgG molecule. The Fc fragment mediates opsonization, antibody dependent cellular cytotoxicity (ADCC), and complement activation through binding to Fc receptors such as CD16, CD32, CD64, and the complement factor C1.
100microl
Verified Reactivity: Human
Antibody Type: Polyclonal
Host Species: Goat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_2820063 (BioLegend Cat. No. 398004)
Structure: Homodimer formed by the constant region of the IgG heavy chain
Distribution: B cells
Ligand/Receptor: CD16, CD32, and CD64
Cell Type: B cells
Biology Area: Immunology
Antigen References: Paul WE. 2003. Fundamental Immunology. Lippincott, Williams & Wilkins, Philadelphia.
Gene ID: NA
UniProt: View information about IgG Fcgamma on UniProt.org
Clone: Poly23980
Regulatory Status: RUO
Isotype: Goat Polyclonal Ig
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.