Biolegend, 398906, Alexa Fluor® 647 anti-human CD66a/b/c/e Antibody, 100tests

The 5B2 monoclonal antibody reacts with the antigen epitopes shared by CD66a, b, c and e, which are members of the CEA (carcinoembryonic antigen) family of the Ig superfamily. CD66 plays a role in hemophilic and heterophilic adhesion. CD66a, also known as CEACAM1 and BGP (biliary glycoprotein), is mainly expressed on granulocytes, binds to CD66a, c, e, and CD62E. CD66c (known as CEACAM6, NCA) is expressed on granulocytes and epithelial cells. The ligands of CD66c are CD66a-e, CD62E and Galectins. CD66d is a major granulocyte receptor mediating recognition and efficient opsonin-independent phagocytosis of CEACAM-binding microorganisms, including Neisseria , Moraxella and Haemophilus species, thus playing an important role in the clearance of pathogens by the innate immune system. CD66e molecule is also called CEA or CEACAM5, and is primarily found on epithelial cells. CD66e binds to CD66a, c and e.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
RRID: AB_2888720 (BioLegend Cat. No. 398905) AB_2888720 (BioLegend Cat. No. 398906)
Structure: Ig superfamily, CEA antigen group, GPI-linked glycoprotein
Distribution: Granulocytes, epithelial cells
Function: Homophilic and heterophilic adhesion
Ligand/Receptor: CD66a-e, CD62E, galectins
Cell Type: Epithelial cells, Granulocytes
Biology Area: Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. Wiley-Liss. A John Wiley & Sons Inc. Publication Chen T, et al. 2001. J Biol Chem. 276:17413. Wang Z, et al. 2016. Mol Clin Oncol. 5:731. Swords DS, et al. 2016. Onco Targets Ther. 9:7459
Gene ID: 108863446801048
UniProt: View information about CD66a/b/c/e on UniProt.org
Clone: 5B2
Regulatory Status: RUO
Other Names: CEACAM1,5,6,8
Isotype: Mouse IgG1, κ