Biolegend, 400128, APC/Cyanine7 Mouse IgG1, κ Isotype Ctrl Antibody, 100tests

The MOPC-21 immunoglobulin has unknown specificity. The isotype of this antibody is mouse IgG1, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
100tests
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The immunoglobulin was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested ICFC - Verified
Recommended Usage: Each lot of this mouse IgG1, κ isotype control antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, use the isotype control at the same concentration as your primary antibody.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), Functional Assay (FA)
Application References(PubMed link indicates BioLegend citation): Carlsten M, et al. 2007. Cancer Res. 67:1317. PubMed Smed-Sörensen A, et al. 2008. Blood 111:5037. PubMed (FA) Bunesmann MM, et al. 2011. Am. J. Respir. Cell. Mol. Biol. Epub. PubMed Matsuyama T, et al. 2005. Infect. Immun. 73:1044. (IF) Correia DV, et al. 2011. Blood 118:992. (FC) PubMed Lian IA, et al. 2011. Placenta. 32:823. PubMed Bufe B, et al. 20015. J Biol Chem. 290:7369. PubMed
Product Citations: Liisborg C, et al. 2022. Acta Ophthalmol. :3. PubMed Vondra S, et al. 2023. Cell Rep. 42:111977. PubMed Obasanmi G, et al. 2023. Cells. 12: . PubMed Cao B, et al. 2022. Nat Commun. 13:6203. PubMed Subhi Y,et al. 2017. Invest Ophthalmol Vis Sci.. 58(12):5242-5250. PubMed Kubo M, et al. 2018. Oncol Rep. 39:417. PubMed Girard MC, et al. 2021. Sci Rep. 11:5511. PubMed Cui L, et al. 2021. Am J Reprod Immunol. 86:e13436. PubMed Helmink BA, et al. 2020. Nature. 577:549. PubMed Stepanenko AA, et al. 2022. Mol Ther Oncolytics. 24:230. PubMed Luo Y, et al. 2021. Nat Commun. 12:3913. PubMed Still C 2nd, et al. 2021. Cell Reports Medicine. 2(7):100343. PubMed Grant R, et al. 2021. Methods Protoc. 4:. PubMed Liisborg C, et al. 2021. Aging (Albany NY). 13:25763. PubMed Mikulkova Z, et al. 2021. Sci Rep. 11:322. PubMed Zhou F, et al. 2018. Cancer Manag Res. 0.727083333. PubMed Tomita T, et al. 2021. Nat Commun. 12:3655. PubMed
RRID: AB_2892538 (BioLegend Cat. No. 400127) AB_2892538 (BioLegend Cat. No. 400128)
Gene ID: NA
Clone: MOPC-21
Regulatory Status: RUO
Isotype: Mouse IgG1, κ