Biolegend, 400130, Alexa Fluor® 647 Mouse IgG1, κ Isotype Ctrl (FC) Antibody, 100tests

The MOPC-21 immunoglobulin has unknown specificity. The isotype of this antibody is mouse IgG1, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
100tests
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The IgG1,κ isotype control solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this mouse IgG1, κ isotype control antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, use the isotype control at the same concentration as your primary antibody. Use our Concentration Lookup tool to find the exact concentrations of your lots of product. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), Functional Assay (FA)
Application References(PubMed link indicates BioLegend citation): Carlsten M, et al. 2007. Cancer Res. 67:1317. PubMed Smed-Sörensen A, et al. 2008. Blood 111:5037. PubMed (FA) Bunesmann MM, et al. 2011. Am. J. Respir. Cell. Mol. Biol. Epub. PubMed Matsuyama T, et al. 2005. Infect. Immun. 73:1044. (IF) Correia DV, et al. 2011. Blood 118:992. (FC) PubMed Lian IA, et al. 2011. Placenta. 32:823. PubMed Bufe B, et al. 20015. J Biol Chem. 290:7369. PubMed
Product Citations: Li C, et al. 2014. Cell Signal. 26:1369. PubMed Mogilenko DA, et al. 2022. Immunol Cell Biol. 100:186. PubMed de Tymowski C et al. 2019. Cell reports. 27(3):762-775 . PubMed Hirano KI, et al. 2021. eLife. 0.416666666666667. PubMed Zhou W, et al. 2020. J Cell Sci. 133:00:00. PubMed Shi Y, et al. 2020. Haematologica. . PubMed Ruetsche D, et al. 2022. Cells. 11:. PubMed Beatson RE, et al. 2021. Cell Rep Med. 2:100473. PubMed Zhou W, et al. 2020. J Cell Sci. . PubMed Che LH, et al. 2021. Cell Discov. 7:80. PubMed Sawanobori Y, et al. 2021. Histochem Cell Biol. 156:133. PubMed Helmink BA, et al. 2020. Nature. 577:549. PubMed Hui HYL, et al. 2021. Curr Protoc. 1:e260. PubMed Gordon-Alonso M,et al. 2017. Nat. Commun. 10.1038/s41467-017-00925-6. PubMed Ohgomori T, et al. 2021. Eur J Neurosci. 54:4740. PubMed Bristol JA, et al. 2022. PLoS Pathog. 18:e1010453. PubMed Huang N, et al. 2020. Genome Biol. 1.03125. PubMed Hsu AY, et al. 2019. Mol Immunol. 112:206. PubMed Meylan P, et al. 2021. Scientific Reports. 11(1):7847. PubMed Navarro-Guerrero E, et al. 2021. Sci Rep. 11:4245. PubMed Tamai K, et al. 2022. Cell Rep Methods. 2:100314. PubMed Baxter AE, et al. 2017. Nat Protoc. 12:2029. PubMed Lamsfus Calle C, et al. 2021. Front Immunol. 12:625712. PubMed Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
RRID: AB_2800436 (BioLegend Cat. No. 400130)
Gene ID: NA
Clone: MOPC-21
Regulatory Status: RUO
Isotype: Mouse IgG1, κ