Biolegend, 400131, Pacific Blue™ Mouse IgG1, κ Isotype Ctrl Antibody, 100μg

The MOPC-21 immunoglobulin has unknown specificity. The isotype of this antibody is mouse IgG1, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
100μg
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: test size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration: µg sizes: 0.5 mg/mLtest sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The IgG1, κ isotype control antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested ICFC - Verified
Recommended Usage: Each lot of this mouse IgG1, ? isotype control antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, use the isotype control at the same concentration as your primary antibody. Use our Concentration Lookup tool to find the exact concentrations of your lots of product. * Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Violet Laser (405 nm)
Application Notes: Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), Functional Assay (FA)
Application References(PubMed link indicates BioLegend citation): Carlsten M, et al. 2007. Cancer Res. 67:1317. PubMed Smed-Sörensen A, et al. 2008. Blood 111:5037. PubMed (FA) Bunesmann MM, et al. 2011. Am. J. Respir. Cell. Mol. Biol. Epub. PubMed Matsuyama T, et al. 2005. Infect. Immun. 73:1044. (IF) Correia DV, et al. 2011. Blood 118:992. (FC) PubMed Lian IA, et al. 2011. Placenta. 32:823. PubMed Bufe B, et al. 20015. J Biol Chem. 290:7369. PubMed
Product Citations: Liisborg C, et al. 2022. Acta Ophthalmol. :3. PubMed Shishkova D, et al. 2022. Int J Mol Sci. 23: . PubMed Gerace D, et al. 2023. Cell Rep Med. 4:100879. PubMed Tyurin-Kuzmin PA, et al. 2018. Sci Data. 5:180196. PubMed Kubo M, et al. 2018. Oncol Rep. 39:417. PubMed Queckborner S, et al. 2020. Stem Cell Res Ther. 11:15. PubMed Masuda T, et al. 2022. iScience. 25:104781. PubMed Bellon A, et al. 2019. Front Mol Neurosci. 11:323. PubMed Liisborg C, et al. 2022. PLoS One. 17:e0269960. PubMed Zou F, et al. 2021. Mol Ther. 29:1794. PubMed Lu Y, et al. 2020. STAR Protoc. 1:100040. PubMed Headland S, et al. 2014. Sci Rep. 4:5237. PubMed Li Q, et al. 2016. Proc Natl Acad Sci U S A. 113: 2672 - 2677. PubMed
RRID: AB_2923473 (BioLegend Cat. No. 400131) AB_2923473 (BioLegend Cat. No. 400151)
Gene ID: NA
Clone: MOPC-21
Regulatory Status: RUO
Isotype: Mouse IgG1, κ