Biolegend, 400132, Alexa Fluor® 488 Mouse IgG1, κ Isotype Ctrl (FC) Antibody, 25tests

The MOPC-21 immunoglobulin has unknown specificity. The isotype of this antibody is mouse IgG1, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
25tests
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this mouse IgG1, κ isotype control antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, use the isotype control at the same concentration as your primary antibody. Use our Concentration Lookup tool to find the exact concentrations of your lots of product. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), Functional Assay (FA)
Application References(PubMed link indicates BioLegend citation): Carlsten M, et al. 2007. Cancer Res. 67:1317. PubMed Smed-Sörensen A, et al. 2008. Blood 111:5037. PubMed (FA) Bunesmann MM, et al. 2011. Am. J. Respir. Cell. Mol. Biol. Epub. PubMed Matsuyama T, et al. 2005. Infect. Immun. 73:1044. (IF) Correia DV, et al. 2011. Blood 118:992. (FC) PubMed Lian IA, et al. 2011. Placenta. 32:823. PubMed Bufe B, et al. 20015. J Biol Chem. 290:7369. PubMed
Product Citations: Chithanathan K, et al. 2022. Front Immunol. 13:996415. PubMed Hu X, et al. 2023. Nat Commun. 14:2020. PubMed Hu X, et al. 2023. Nat Biotechnol. . PubMed Sulaj A, et al. 2022. J Clin Endocrinol Metab. 107:2167. PubMed Chithanathan K, et al. 2022. Cells. 11: . PubMed Li S, et al. 2022. Nat Commun. 13:7371. PubMed Vondra S, et al. 2023. Cell Rep. 42:111977. PubMed Werner NT, et al. 2023. Commun Biol. 6:177. PubMed PÉcot J, et al. 2016. Cell Rep. 17:3347-3358. PubMed Chen Y, et al. 2019. Sci Rep. 9:5624. PubMed Slamecka J, et al. 2017. Cell Cycle. 17:330. PubMed Zhu S, et al. 2022. Front Pharmacol. 13:996635. PubMed Menck K, et al. 2015. J Mol Cell Biol. 7:143. PubMed Giannella A, et al. 2021. Cardiovasc Diabetol. 20:77. PubMed Zobel MJ, et al. 2020. J Immunother Cancer. 8:00. PubMed Hopf A, et al. 2022. Bioengineering (Basel). 9:. PubMed Shadrin N, et al. 2015. Exp Cell Res. 330:223. PubMed Morelli AE, et al. 2020. Cell Rep. 30:3448. PubMed Ji S, et al. 2021. Clin Sci (Lond). 135:1127. PubMed Ubellacker JM, et al. 2020. Nature. 585:113. PubMed Maleki KT, et al. 2021. Cell Reports Medicine. 2(3):100220. PubMed Hwang B, et al. 2021. Nat Methods. 18:903. PubMed Dubé N, et al. 2008. Cell Signal. 20:1608. PubMed Tamai K, et al. 2022. Cell Rep Methods. 2:100314. PubMed Baxter AE, et al. 2017. Nat Protoc. 12:2029. PubMed Farhat AM, et al. 2021. Cell Reports. 35(4):109044. PubMed Piirainen S, et al. 2021. Glia. 69:2459. PubMed Tomita T, et al. 2021. Nat Commun. 12:3655. PubMed Gerace D, et al. 2021. Stem Cell Research. 53:102371. PubMed Lynskey N, et al. 2017. PLoS Pathog.. 10.1371/journal.ppat.1006493. PubMed Shiba T, et al. 2019. J Allergy Clin Immunol. 144:1438. PubMed Chijimatsu R, et al. 2022. Regen Ther. 21:52. PubMed Chithanathan K, et al. 2022. Biology (Basel). 11:. PubMed
RRID: AB_2890263 (BioLegend Cat. No. 400132) AB_2890263 (BioLegend Cat. No. 400129)
Gene ID: NA
Clone: MOPC-21
Regulatory Status: RUO
Isotype: Mouse IgG1, κ