Biolegend, 400147, PerCP Mouse IgG1, κ Isotype Ctrl Antibody, 25tests

The MOPC-21 immunoglobulin has unknown specificity. The isotype of this antibody is mouse IgG1, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
25tests
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The immunoglobulin was purified by affinity chromatography, and conjugated with PerCP under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested ICFC - Verified
Recommended Usage: Each lot of this mouse IgG1, ? isotype control antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, use the isotype control at the same concentration as your primary antibody. Use our Concentration Lookup tool to find the exact concentrations of your lots of product. * PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), Functional Assay (FA)
Application References(PubMed link indicates BioLegend citation): Carlsten M, et al. 2007. Cancer Res. 67:1317. PubMed Smed-Sörensen A, et al. 2008. Blood 111:5037. PubMed (FA) Bunesmann MM, et al. 2011. Am. J. Respir. Cell. Mol. Biol. Epub. PubMed Matsuyama T, et al. 2005. Infect. Immun. 73:1044. (IF) Correia DV, et al. 2011. Blood 118:992. (FC) PubMed Lian IA, et al. 2011. Placenta. 32:823. PubMed Bufe B, et al. 20015. J Biol Chem. 290:7369. PubMed
Product Citations: Gurses K, et al. 2014. Atherosclerosis. 237:784. PubMed Hu X, et al. 2023. Nat Commun. 14:2020. PubMed Hu X, et al. 2023. Nat Biotechnol. . PubMed Imai H, et al. 2023. iScience. 26:106822. PubMed Wohlgemuth L, et al. 2022. Cells. 11: . PubMed Cao B, et al. 2022. Nat Commun. 13:6203. PubMed Beatson RE, et al. 2021. Cell Rep Med. 2:100473. PubMed Sawasdee N, et al. 2022. Int J Mol Med. 49:. PubMed Tan L, et al. 2016. Sci Rep. 6: 25314. PubMed Nieto C, et al. 2018. Front Immunol. 9:31. PubMed
RRID: AB_893679 (BioLegend Cat. No. 400147) AB_893679 (BioLegend Cat. No. 400148)
Gene ID: NA
Clone: MOPC-21
Regulatory Status: RUO
Isotype: Mouse IgG1, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.