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BRAND / VENDOR: Biolegend

Biolegend, 400220, APC Mouse IgG2a, κ Isotype Ctrl Antibody, 100μg

CATALOG NUMBER: 400220
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Product Description

The MOPC-173 immunoglobulin has unknown specificity. The isotype of this antibody is mouse IgG2a, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat, and human tissues.
100μg
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The immunoglobulin was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), Functional Assay (FA).
Product Citations: Ho J, et al. 2016. PLoS Biol. 14:e2000117. PubMed Hu X, et al. 2023. Nat Biotechnol. . PubMed Oh J, et al. 2023. Nat Commun. 14:3278. PubMed Martínez-Sánchez MV, et al. 2021. Cancers (Basel). 13: . PubMed Chithanathan K, et al. 2022. Cells. 11: . PubMed Market M, et al. 2022. Int J Mol Sci. 23: . PubMed Yan L, et al. 2021. Front Cell Neurosci. 15:750373. PubMed Ruetsche D, et al. 2022. Cells. 11:. PubMed Li J, et al. 2021. Front Cell Dev Biol. 9:737242. PubMed Li J, et al. 2022. Int J Mol Sci. 23:. PubMed Market M, et al. 2020. Front Immunol. 1.127083333. PubMed Shan X et al. 2017. eLife. 6 pii: e25306. PubMed Coelho PP, et al. 2022. Nat Commun. 13:3812. PubMed Yamasaki S, et al. 2022. iScience. 25:103657. PubMed Witkowski MT, et al. 2020. Cancer Cell. 37:867. PubMed Tom EC, et al. 2020. Mol Cell Biol. 40:00:00. PubMed Wegner J, et al. 2021. Sci Rep. 11:14983. PubMed Perera MR, et al. 2022. Int J Mol Sci. 23:. PubMed Lan X, et al. 2022. Nat Commun. 13:4601. PubMed Hilt ZT, et al. 2019. JCI Insight. 4:5. PubMed Samuel RM, et al. 2020. Cell Stem Cell. 27:876. PubMed Lewis D, et al. 2015. Proc Natl Acad Sci U S A. 112:2693. PubMed
RRID: AB_326468 (BioLegend Cat. No. 400219) AB_326468 (BioLegend Cat. No. 400220)
Gene ID: NA
Clone: MOPC-173
Regulatory Status: RUO
Isotype: Mouse IgG2a, κ
Q: IgG2a gene is deleted in some mouse strains, which ones are they?
A: IgG2a gene is deleted in C57Bl/6, C57Bl/10, SJL, and NOD mice. It is replaced with IgG2c gene instead.


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