Biolegend, 400232, PE/Cyanine7 Mouse IgG2a, κ Isotype Ctrl Antibody, 100tests

The MOPC-173 immunoglobulin has unknown specificity. The isotype of this antibody is mouse IgG2a, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat, and human tissues.
100tests
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.test size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: µg sizes: 0.2 mg/mLtest sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested ICFC - Verified
Recommended Usage: Each lot of this mouse IgG2a, ? isotype control antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, use the isotype control at the same concentration as your primary antibody.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), Functional Assay (FA).
Product Citations: Liisborg C, et al. 2022. Acta Ophthalmol. :3. PubMed Campbell KA, et al. 2023. Commun Biol. 6:264. PubMed Iancu O, et al. 2023. Mol Ther Nucleic Acids. 31:105. PubMed Cao B, et al. 2022. Nat Commun. 13:6203. PubMed Subhi Y,et al. 2017. Invest Ophthalmol Vis Sci.. 58(12):5242-5250. PubMed Nishihara H, et al. 2020. Fluids Barriers CNS. 17:3. PubMed Girard MC, et al. 2021. Sci Rep. 11:5511. PubMed Hegde S, et al. 2020. Cancer Cell. 37(3):289-307. PubMed Lindenbergh MFS, et al. 2020. J Extracell Vesicles. 1798606:9. PubMed Song S, et al. 2021. Front Immunol. 12:705140. PubMed Nishihara H, et al. 2021. STAR Protocols. 2(2):100563. PubMed
RRID: AB_10697032 (BioLegend Cat. No. 400253) AB_2864288 (BioLegend Cat. No. 400231) AB_326480 (BioLegend Cat. No. 400232) AB_10693906 (BioLegend Cat. No. 400254)
Gene ID: NA
Clone: MOPC-173
Regulatory Status: RUO
Isotype: Mouse IgG2a, κ
Q: IgG2a gene is deleted in some mouse strains, which ones are they?
A: IgG2a gene is deleted in C57Bl/6, C57Bl/10, SJL, and NOD mice. It is replaced with IgG2c gene instead.