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BRAND / VENDOR: Biolegend

Biolegend, 400313, PE Mouse IgG2b, κ Isotype Ctrl Antibody, 25tests

CATALOG NUMBER: 400313
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Product Description

The MPC-11 immunoglobulin has unknown specificity. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
25tests
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).µg sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The immunoglobulin was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: µg sizes: 0.2 mg/mLtest sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The immunoglobulin solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested ICFC - Verified
Recommended Usage: Each lot of this mouse IgG2b, ? isotype control antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, use the isotype control at the same concentration as your primary antibody. Use our Concentration Lookup tool to find the exact concentrations of your lots of product.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), Functional Assay (FA).
Application References(PubMed link indicates BioLegend citation): Smed-Sörensen A, et al. 2008. Blood 111:5037. (FA) PubMed Podolin PL, et al. 2008. J. Immunol. 180:7989. (FC) PubMed
Product Citations: Hu X, et al. 2023. Nat Biotechnol. . PubMed Wang Y, et al. 2023. iScience. 26:106630. PubMed Gu T, et al. 2023. Front Immunol. 14:1145028. PubMed Oreskovic E, et al. 2022. Proc Natl Acad Sci U S A. 119:. PubMed Chen E, et al. 2021. Gastroenterology. 160:1709. PubMed Jiao Q, et al. 2020. Br J Dermatol. 182:648. PubMed Mogilenko DA, et al. 2022. Immunol Cell Biol. 100:186. PubMed Kuczynski EA, et al. 2022. EMBO Mol Med. 14:e15816. PubMed Linke D, et al. 2022. Int J Mol Sci. 24: . PubMed He Y, et al. 2023. J Transl Med. 21:25. PubMed Ameres S, et al. 2014. J Immunol. 192:5894. PubMed Fielding CA et al. 2017. eLife. 6 pii: e22206. PubMed Hu K, et al. 2022. ACS Omega. 7:334. PubMed Santiago L, et al. 2020. Cell Rep. 107847:32. PubMed Kuang YL, et al. 2019. Stem Cell Res. 37:101434. PubMed Oh JH, et al. 2019. Cancer Immunol Res. 0.697222222. PubMed Masumoto K, et al. 2022. Sci Rep. 12:10351. PubMed Yang GY, et al. 2020. Vet Res. 51:26. PubMed Glaubitz J, et al. 2022. Nat Commun. 13:4502. PubMed Guan H, et al. 2016. Sci Rep. 6:35651. PubMed Su W, et al. 2022. Front Immunol. 13:952338. PubMed Lee WS, et al. 2020. Arthritis Rheumatol. 72:1493. PubMed Tang S, et al. 2021. Front Cell Dev Biol. 9:670913. PubMed Berger V, et al. 2013. Protein Eng Des Sel. 26:581. PubMed Sawanobori Y, et al. 2021. Histochem Cell Biol. 156:133. PubMed Herter JM, et al. 2022. Strahlenther Onkol. Online ahead of print. PubMed Ibuki M, et al. 2019. Regen Ther. 0.551388889. PubMed Wu Y, et al. 2021. Nat Commun. 12:2346. PubMed Xu Z, et al. 2016. Nat Commun. 7:10728. PubMed Mendoza-Cabrera MI, et al. 2019. Innate Immun. :1753425919864658. PubMed Sendler M, et al. 2020. Gastroenterology. 158:253. PubMed Bouleau A, et al. 2022. J Nucl Med. 63:1259. PubMed Yoshino H, et al. 2021. Curr Issues Mol Biol. 43:153. PubMed Grant R, et al. 2021. Methods Protoc. 4:. PubMed Pucci M, et al. 2021. Int J Mol Sci. 22:. PubMed Ahn A, et al. 2021. Cancers (Basel). 13:. PubMed Kim N, et al. 2020. Cancers (Basel). 12:. PubMed Tsuchiya K, et al. 2021. Oncoimmunology. 10:1962656. PubMed Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed Venet F, et al. 2017. J Immunol. 10.4049/jimmunol.1700127. PubMed Patel C, et al. 2022. Curr Protoc. 2:e400. PubMed Coleman LG, et al. 2018. PLoS One. 13:e0195335. PubMed Kawamoto E, et al. 2021. Biomedicines. 9:. PubMed Strait AA, et al. 2021. Commun Biol. 4:1005. PubMed Rosenbaum SR, et al. 2021. Cell Rep. 37:110085. PubMed
RRID: AB_2894969 (BioLegend Cat. No. 400311) AB_2894969 (BioLegend Cat. No. 400313) AB_2894969 (BioLegend Cat. No. 400312) AB_2894969 (BioLegend Cat. No. 400314)
Gene ID: NA
Clone: MPC-11
Regulatory Status: RUO
Isotype: Mouse IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.


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Tony Tang

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