Biolegend, 400325, PE/Cyanine7 Mouse IgG2b, κ Isotype Ctrl Antibody, 25tests

The MPC-11 immunoglobulin has unknown specificity. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
25tests
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested ICFC - Verified
Recommended Usage: Each lot of this mouse IgG2b, κ isotype control antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, use the isotype control at the same concentration as your primary antibody.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), Functional Assay (FA).
Application References(PubMed link indicates BioLegend citation): Smed-Sörensen A, et al. 2008. Blood 111:5037. (FA) PubMed Podolin PL, et al. 2008. J. Immunol. 180:7989. (FC) PubMed
Product Citations: Bhatt RS, et al. 2021. Cancer Immunol Res. 9:156. PubMed Zenke S, et al. 2022. Nat Commun. 13:6459. PubMed Fu B et al. 2017. Immunity. 47(6):1100-1113 . PubMed Kitajima S, et al. 2019. Cancer Discov. 9:34. PubMed Cui L, et al. 2021. Am J Reprod Immunol. 86:e13436. PubMed Campisi M, et al. 2020. Front Immunol. 1.909722222. PubMed Helmink BA, et al. 2020. Nature. 577:549. PubMed Cañadas I, et al. 2018. Nat Med. 24:1143. PubMed Yoshida R, et al. 2022. Cancer Res. :. PubMed Molina MS, et al. 2021. Front Immunol. 12:699128. PubMed Elgamal S, et al. 2021. JCI Insight. 6:. PubMed Zhang Q, et al. 2018. J Immunol Res. 2017:6915912. PubMed
RRID: AB_2923259 (BioLegend Cat. No. 400325) AB_2923259 (BioLegend Cat. No. 400326)
Gene ID: NA
Clone: MPC-11
Regulatory Status: RUO
Isotype: Mouse IgG2b, κ