Biolegend, 400342, Brilliant Violet 421™ Mouse IgG2b, κ Isotype Ctrl Antibody, 100tests

The MPC-11 immunoglobulin has unknown specificity. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
100tests
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The immunoglobulin was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested ICFC - Verified
Recommended Usage: Each lot of this mouse IgG2b, ? isotype control antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, use the isotype control at the same concentration as your primary antibody. Use our Concentration Lookup tool to find the exact concentrations of your lots of product. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), Functional Assay (FA).
Application References(PubMed link indicates BioLegend citation): Smed-Sörensen A, et al. 2008. Blood 111:5037. (FA) PubMed Podolin PL, et al. 2008. J. Immunol. 180:7989. (FC) PubMed
Product Citations: Hu X, et al. 2023. Nat Commun. 14:2020. PubMed Imai H, et al. 2023. iScience. 26:106822. PubMed Lilianty J, et al. 2020. Stem Cell Research. 72.59930556. PubMed Glaubitz J, et al. 2022. Nat Commun. 13:4502. PubMed Nur Patria Y, et al. 2020. Stem Cell Research. 42:101689.. PubMed Mohan N, et al. 2022. Front Immunol. 13:918528. PubMed Eriksson E, et al. 2019. J Immunol. 202:787. PubMed Schütz C, et al. 2017. Leukemia. 10.1038/leu.2017.9. PubMed Howden S et al. 2019. Stem Cell Res. 1728:53:00 . PubMed Zhang X, et al. 2021. Nat Commun. 12:4536. PubMed Hosseini Far H et al. 2019. Stem Cell Res. 1727:49:00 . PubMed Maurer DM, et al. 2020. Cancer Immunol Res. 8:1554. PubMed Weidner T, et al. 2021. Nat Protoc. 16:3210. PubMed
RRID: AB_10898160 (BioLegend Cat. No. 400341) AB_10898160 (BioLegend Cat. No. 400342)
Gene ID: NA
Clone: MPC-11
Regulatory Status: RUO
Isotype: Mouse IgG2b, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.