Biolegend, 400402, Purified Rat IgG1, κ Isotype Ctrl Antibody, 500μg

The isotype of RTK2071 immunoglobulin is rat IgG1, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
500μg
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Trinitrophenol + KLH
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The IgG1, κ isotype control solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested ICFC, ICC, IHC, IP, WB - Reported in the literature, not verified in house
Recommended Usage: Each lot of this rat IgG1, κ isotype control antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control, and the purity is greater than 95% by SDS-PAGE. Use at concentrations comparable to those of the specific antibody of interest. Use our Concentration Lookup tool to find the exact concentrations of your lots of product.
Application Notes: The RTK2071 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, and immunofluorescence microscopy. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 400414) as negative control1. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 400432) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).
Application References(PubMed link indicates BioLegend citation): Riemann M, et al. 2005. J. Immunol. 175:3560. PubMed Wondimu Z, et al. 2010. Am. J. Pathol. 177:2334. PubMed
Product Citations: Chiche A et al. 2017. Cell stem cell. 20(3):407-414 . PubMed Wondimu Z, et al. 2010. Am J Pathol. 177:2334. PubMed Akhtar MN, et al. 2022. MBio. 13:e0330921. PubMed Shang P, et al. 2023. Nat Commun. 14:2615. PubMed Yang H, et al. 2022. Theranostics. 12:7668. PubMed Ondigo BN, et al. 2018. Am J Trop Med Hyg. 98:1770. PubMed Oleinika K, et al. 2018. Nat Commun. 9:684. PubMed Wang X, et al. 2020. Signal Transduct Target Ther. 5:35. PubMed Tada R, et al. 2021. Int Immunopharmacol. 101:108280. PubMed Wan Jun Gan et al. 2018. Cell reports. 24(11):2819-2826 . PubMed Osborne SE, et al. 2017. Cell Microbiol. 19:3. PubMed Pedras-Vasconcelos J, et al. 2008. J Immunol. 180:8231. PubMed Uehara H, et al. 2021. eLife. 10:00. PubMed Riemann M, et al. 2005. J Immunol. 175:3560. PubMed Lin A, et al. 2022. Bioeng Transl Med. 7:e10314. PubMed Lagarrigue F, et al. 2018. Blood Adv. 1.720833333. PubMed Wang Y, et al. 2021. Front Immunol. 11:601601. PubMed Alam IS, et al. 2020. Cancer Res. 80:4780. PubMed Galeano Nio JL, et al. 2020. eLife. 9:00. PubMed Wichaiyo S, et al. 2019. Haematologica. 104:1648. PubMed Zhang M, et al. 2022. Bioengineered. 13:10072. PubMed
RRID: AB_326507 (BioLegend Cat. No. 400401) AB_326508 (BioLegend Cat. No. 400402)
Gene ID: NA
Clone: RTK2071
Regulatory Status: RUO
Isotype: Rat IgG1, κ