Biolegend, 400412, APC Rat IgG1, κ Isotype Ctrl Antibody, 100μg

The isotype of RTK2071 immunoglobulin is rat IgG1, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
100μg
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Trinitrophenol + KLH
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest. Use our Concentration Lookup tool to find the exact concentrations of your lots of product.
Excitation Laser: Red Laser (633 nm)
Application Notes: The RTK2071 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, and immunofluorescence microscopy. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 400414) as negative control1. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 400432) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).
Application References(PubMed link indicates BioLegend citation): Riemann M, et al. 2005. J. Immunol. 175:3560. PubMed Wondimu Z, et al. 2010. Am. J. Pathol. 177:2334. PubMed
Product Citations: Jiang X, et al. 2023. Adv Healthc Mater. 12:e2203397. PubMed Schwarz A, et al. 2023. Front Immunol. 14:1038689. PubMed Vijver SV, et al. 2023. Front Immunol. 13:1100730. PubMed Chen X, et al. 2021. Theranostics. 11:4655. PubMed Periasamy S, et al. 2017. Nat Commun. 8:15564. PubMed Choi H et al. 2019. Cell Rep. 27(3):806-819 . PubMed Dolgova EV, et al. 2022. Int J Mol Sci. 23:. PubMed Santos R, et al. 2017. Stem Cell Reports. 1.553472222. PubMed Zhang D, et al. 2020. Signal Transduct Target Ther. 5:24. PubMed Hegde S, et al. 2020. Cancer Cell. 37(3):289-307. PubMed Niemann J, et al. 2019. Nat Commun. 10:3236. PubMed Martínez‐López M et al. 2019. Immunity. 50(2):446-461 . PubMed Yang B, et al. 2022. Bioengineered. 13:2685. PubMed Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed De Vlaminck K, et al. 2021. Front Immunol. 12:777524. PubMed Molina MS, et al. 2021. Front Immunol. 12:699128. PubMed Zhang D, et al. 2020. Signal Transduct Target Ther. 5:24. PubMed Dai X, et al. 2021. Molecular Cell. 81(11):2317-2331.e6. PubMed Patel C, et al. 2022. Curr Protoc. 2:e400. PubMed Ding X, et al. 2021. Front Immunol. 12:667136. PubMed
RRID: AB_326517 (BioLegend Cat. No. 400411) AB_326518 (BioLegend Cat. No. 400412)
Gene ID: NA
Clone: RTK2071
Regulatory Status: RUO
Isotype: Rat IgG1, κ