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BRAND / VENDOR: Biolegend

Biolegend, 400506, FITC Rat IgG2a, κ Isotype Ctrl Antibody, 200μg

CATALOG NUMBER: 400506
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Product Description

The RTK2758 immunoglobulin reacts with KLH. The isotype of this antibody is rat IgG2a, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
200μg
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Trinitrophenol + KLH
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The immunoglobulin was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), and Functional Assay (FA).
Application References(PubMed link indicates BioLegend citation): Nishimoto H, et al. 2005. Blood 106:4241. Seach N, et al. 2008. J. Immunol. 180:5384. PubMed Jiang P, et al. 1999. J. Biol. Chem. 274:559. (FA)
Product Citations: White Z, et al. 2022. Blood Cells Mol Dis. 97:102688. PubMed Suzuki Y, et al. 2023. Front Immunol. 14:1089905. PubMed Pioli KT, et al. 2023. iScience. 26:106223. PubMed Hattori Y, et al. 2022. J Neurosci. 42:362. PubMed Ding L, et al. 2023. J Clin Invest. 133: . PubMed Urata S, et al. 2023. Microbiol Immunol. 67:105. PubMed Ye B, et al. 2022. Cell Mol Immunol. 19:619. PubMed Reglero-Real N, et al. 2021. Immunity. :. PubMed Miao L, et al. 2020. Theranostics. 0.7625. PubMed Iwasaki Y, et al. 2016. PLoS One. 11: 0158282. PubMed Chen X, et al. 2021. Theranostics. 11:4655. PubMed Go DM, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:715. PubMed Zhuang Z, et al. 2021. J Exp Med. 218:00:00. PubMed Blank A, et al. 2021. J Pathol. 253:160. PubMed She L, et al. 2021. JCI Insight. 6:e143509. PubMed McCreedy DA et al. 2018. eNeuro. 5(4) pii: ENEURO. PubMed Wosczyna MN et al. 2019. Cell Rep. 27(7):2029-2035 . PubMed Niemann J, et al. 2019. Nat Commun. 10:3236. PubMed Jones KB et al. 2018. Cell stem cell. 24(1):183-192 . PubMed Wang X, et al. 2018. Cell Death Dis. 9:578. PubMed Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed Jamali A, et al. 2020. Cell Reports. 32(9):108099. PubMed Park JS, et al. 2022. Arthritis Res Ther. 24:254. PubMed Benhamron S, et al. 2012. PLoS One. 7:e35602. PubMed Hori Y, et al. 2022. Front Cardiovasc Med. 9:981088. PubMed Deng P, et al. 2021. Cell Stem Cell. 28(6):1057-1073.e7. PubMed Deng Q, et al. 2021. Gut Microbes. 13:1. PubMed Tomimatsu M, et al. 2022. Sci Rep. 12:16656. PubMed Kaji T, et al. 2012. J Exp Med. 80:3642. PubMed Patel C, et al. 2022. Curr Protoc. 2:e400. PubMed Yang C, et al. 2019. J Transl Med. 1.004166667. PubMed Schroder WA, et al. 2019. Sci Rep. 9:12421. PubMed Jiang H, et al. 2022. Cell Death Dis. 13:183. PubMed Albaghdadi AJH, et al. 2019. Sci Rep. 9:6528. PubMed Hong Y, et al. 2019. J Extracell Vesicles. 8:1670893. PubMed Zhang M, et al. 2019. Autophagy. 1.423611111. PubMed Deniset JF et al. 2019. Immunity. 51(1):131-140 . PubMed
RRID: AB_2736919 (BioLegend Cat. No. 400505) AB_2736919 (BioLegend Cat. No. 400506)
Gene ID: NA
Clone: RTK2758
Regulatory Status: RUO
Isotype: Rat IgG2a, κ


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