Biolegend, 400525, Alexa Fluor® 488 Rat IgG2a, κ Isotype Ctrl Antibody, 100μg

The RTK2758 immunoglobulin reacts with KLH. The isotype of this antibody is rat IgG2a, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
100μg
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Trinitrophenol + KLH
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), and Functional Assay (FA).
Application References(PubMed link indicates BioLegend citation): Nishimoto H, et al. 2005. Blood 106:4241. Seach N, et al. 2008. J. Immunol. 180:5384. PubMed Jiang P, et al. 1999. J. Biol. Chem. 274:559. (FA)
Product Citations: Ojeda-Fernández L, et al. 2016. PLoS One. 12: 1005935. PubMed Ho J, et al. 2016. PLoS Biol. 14:e2000117. PubMed Neufert C, et al. 2020. Nature Protocols. 16(1):61-85. PubMed Pelon F, et al. 2020. Nat Commun. 0.738888889. PubMed Mizutani T, et al. 2023. iScience. 26:106081. PubMed Evans JG, et al. 2023. Viruses. 15:. PubMed Connor T, et al. 2022. Methods Mol Biol. 2475:47. PubMed Xie L, et al. 2022. Cell Mol Gastroenterol Hepatol. 14:435. PubMed El-Naccache DW, et al. 2022. Cell Rep. 40:111150. PubMed Lokmic Z, et al. 2015. J Vis Exp. 99: 52691. PubMed Davis F, et al. 2015. Proc Natl Acad Sci U S A. 112:5827. PubMed Ruetsche D, et al. 2022. Cells. 11:. PubMed Zhuang Z, et al. 2021. J Exp Med. 218:00:00. PubMed He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed Eichin D, et al. 2015. PLoS One. 10: 0134721. PubMed Thiriot A et al. 2017. BMC biology. 15(1):45 . PubMed Bota-Rabassedas N, et al. 2021. Cell Reports. 35(3):109009. PubMed Takizawa S, et al. 2022. Mol Med. 28:91. PubMed Sakamoto K, et al. 2021. Immunity. 54:2321. PubMed Weigelin B, et al. 2021. Nat Commun. 12:5217. PubMed
RRID: AB_2864283 (BioLegend Cat. No. 400525)
Gene ID: NA
Clone: RTK2758
Regulatory Status: RUO
Isotype: Rat IgG2a, κ