Biolegend, 400664, GoInVivo™ Purified Rat IgG2b, κ Isotype Ctrl Antibody, 500mg

The isotype of RTK4530 immunoglobulin is rat IgG2b, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
500mg
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Trinitrophenol + KLH
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 1.0 EU/mg of the protein (< 0.1 pg/µg of the protein) as determined by the LAL test.
Preparation: The GoInVivo™ antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This GoInVivo™ solution contains no preservative; handle under aseptic conditions.
Application: FC - Quality tested ICFC, ICC, IHC, IP, WB, FA - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. For in vivo and in vitro applications, we recommend to perform a pilot experiment to determine the optimal concentration to use for each particular experiment.
Application Notes: The RTK4530 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, and immunofluorescence microscopy. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 400643, 400644, 400671, 400672, 400675, and 400676) as negative control.
Additional Product Notes: GoInVivo™ products are guaranteed to be pathogen-free based on the IDEXX BioResearch IMPACT test via PCR. For a full listing of pathogens tested, visit the GoInVivo™ Webpage. View more applications data for this product in our Scientific Poster Library.
Application References(PubMed link indicates BioLegend citation): Cervantes-Barragan L, et al. 2007. Blood 109:1131. Zeiser R, et al. 2007. Blood 109:2225. Sasaki K, et al. 2008. J. Immunol. 181:104. PubMed Duan J, et al. 2008. P. Natl. Acad. Sci. USA 105:5183. PubMed Yi H, et al. 2009. Blood 113:5819. PubMed Schafeer JS, et al. 2010. J. Leukocyte Biol. 87:301. PubMed Lei GS, et al. 2015. Infect Immun. 83:572. PubMed Richards J, et al. 2015. Mol Cell Cardiol. 79:21. PubMed
Product Citations: MacLean AJ, et al. 2024. J Exp Med. :221. PubMed Chen X, et al. 2022. J Extracell Vesicles. 11:e12279. PubMed Adamson SE, et al. 2018. Arterioscler Thromb Vasc Biol. 38:1020. PubMed Habib S, et al. 2018. Infect Immun. 86:e00019. PubMed Yamazaki K, et al. 2022. Sci Rep. 12:3242. PubMed
RRID: AB_3097011 (BioLegend Cat. No. 400666) AB_3097011 (BioLegend Cat. No. 400667) AB_3097011 (BioLegend Cat. No. 400668) AB_3097011 (BioLegend Cat. No. 400663) AB_3097011 (BioLegend Cat. No. 400664) AB_3097011 (BioLegend Cat. No. 400665)
Gene ID: NA
Clone: RTK4530
Regulatory Status: RUO
Isotype: Rat IgG2b, κ