Biolegend, 400676, Ultra-LEAF™ Purified Rat IgG2b, κ Isotype Ctrl Antibody, 100mg

The isotype of RTK4530 immunoglobulin is rat IgG2b, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
100mg
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Trinitrophenol + KLH
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: FC - Quality tested ICFC, ICC, IHC, IP, WB, FA - Reported in the literature, not verified in house
Recommended Usage: Each lot of this rat IgG2b, κ isotype control antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control, and the purity is greater than 95% by SDS-PAGE. Use at concentrations comparable to those of the specific antibody of interest.
Application Notes: The RTK4530 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, and immunofluorescence microscopy. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 400643, 400644, 400671, 400672, 400675, and 400676) as negative control.
Additional Product Notes: View more applications data for this product in our Scientific Poster Library. Watch a Scientific Poster video of this product.
Application References(PubMed link indicates BioLegend citation): Cervantes-Barragan L, et al. 2007. Blood 109:1131. Zeiser R, et al. 2007. Blood 109:2225. Sasaki K, et al. 2008. J. Immunol. 181:104. PubMed Duan J, et al. 2008. P. Natl. Acad. Sci. USA 105:5183. PubMed Yi H, et al. 2009. Blood 113:5819. PubMed Schafeer JS, et al. 2010. J. Leukocyte Biol. 87:301. PubMed Lei GS, et al. 2015. Infect Immun. 83:572. PubMed Richards J, et al. 2015. Mol Cell Cardiol. 79:21. PubMed
Product Citations: Akhtar MN, et al. 2022. MBio. 13:e0330921. PubMed Zhu S, et al. 2023. J Nanobiotechnology. 21:81. PubMed Mishra M, et al. 2023. Cell Death Dis. 14:331. PubMed Stieg DC, et al. 2023. Cancer Res Commun. 1200:3. PubMed Alam A, et al. 2022. Cancer Cell. 40:153. PubMed Ocaña-Guzman R, et al. 2022. Cells. 11: . PubMed Sodir NM, et al. 2020. Cancer Discov. 0.825. PubMed Neupane AS, et al. 2020. Cell. 183(1):110-125.e11. PubMed Rocha-Resende C, et al. 2021. J Mol Cell Cardiol. 157:98. PubMed Shibuya M, et al. 2021. iScience. 24:103131. PubMed Bonamichi-Santos R, et al. 2021. Front Immunol. 12:655958. PubMed Morrissey MA, et al. 2020. Immunity. 53(2):290-302.e6. PubMed Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed Kerdidani D, et al. 2022. J Exp Med. 219:. PubMed
RRID: AB_11147763 (BioLegend Cat. No. 400643) AB_11149687 (BioLegend Cat. No. 400644) AB_11149687 (BioLegend Cat. No. 400671) AB_11149687 (BioLegend Cat. No. 400672) AB_11149687 (BioLegend Cat. No. 400675) AB_11149687 (BioLegend Cat. No. 400676)
Gene ID: NA
Clone: RTK4530
Regulatory Status: RUO
Isotype: Rat IgG2b, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.